# This protocol is for full system flush using Sig H2O and 70% ETOH #step 1 Flush SS, intake, Exhaust Requires flush puck in CC #final step pushes flush back through intake so use a separate flush #for this step loadFlush.to:CC SSV.connect :CBV CTV.to:intake Intake.open #flush intake SS.fill SSV.to :air #flush port 1 SS.to 10 SSV.to:exhaust #flush port 4 Exhaust.open SS.empty SSV.to:CBV SS.fill SSV.to :air #flush port 1 SS.to 10 SSV.to:exhaust #flush port 4 Exhaust.open SS.empty CTV.to :air #air out flushed lines SSV.to :CBV SS.fill CTV.to:intake SS.empty CTV.to :air SS.fill SSV.to :air SS.to 10 SSV.to :exhaust SS.empty Intake.close Exhaust.close #step 2 Flush CS and CSV requires flush puck in CC already loaded in step 1 CSV.to :flush CS.to 10 CSV.to :air2 # flush air2 (2) CS.empty CSV.to :flush CS.to 10 CSV.to :waste2 # flush waste1 (1) CS.empty CSV.to :air2 # this step to air out air2 CS.fill CSV.to :waste2 CSV.to :flush CS.to 10 CSV.connect :CBV # flush port 3 thru CTV air CTV.to :air CS.empty CSV.to :air2 CS.fill CSV.to :CBV CS.empty # airs out port 3 thru CTV CSV.to :flush CS.fill CSV.to :waste1 # flush out port 4 CS.empty CSV.to :air2 CS.fill CSV.to :waste1 # air out port 4 waste1 CS.empty CSV.to :flush CS.fill CSV.to :air1 # flush out port 6 CS.empty CSV.to :air2 CS.fill CSV.to :air1 # air out port 6 CS.empty CSV.to :flush CS.fill CSV.connect :CTV # flush out port 7 thru CBV waste2 CBV.to :waste2 CS.empty CSV.to :air1 CS.fill CSV.to :CTV # air out port 7 thru CBV CS.empty CSV.to :flush CS.fill CSV.to :CSR # flush out port 8 CSR CSR.to :air CS.empty CSV.to :air1 # air out port 8 thru CSR CS.fill CSV.to CSR CS.empty #step 3 Flush CSR CSV.to :CSR CSR.to :lysis # valve 0 CS.fill CSV.to :waste2 CS.empty CSV.to :CSR CSR.to :fix # valve 1 CS.fill CSV.to :waste2 CS.empty CSV.to :CSR CSR.to :AqMeOH # valve 2 CS.fill CSV.to :waste2 CS.empty CSR.to :diluent # valve 3 CS.fill CSV.to :waste2 CS.empty CSR.to 4 # valve 4 CS.fill CSV.to :waste2 CS.empty CSR.to 5 # valve 5 CS.fill CSV.to :waste2 CS.empty CSR.to :kill # valve 6 CS.fill CSV.to :waste2 CS.empty CSR.to :flush # valve 7 CS.fill CSV.to :waste2 CS.empty CSV.to :flush CS.fill CSV.to :CSR CSR.to :air # flush out CSR CS.empty CSV.to :air1 CS.fill CSV.to :CSR CS.empty # air out CSR # step 4 flush CBV flush puck required in CC still loaded from step 2 CSV.to :flush CS.fill CSV.to :CTV CBV.to :waste1 # start waste1 flush CS.empty CSV.to :flush CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty # end waste1 flush # PRV gets flushed later on # PSV gets flushed later on CSV.to :flush CS.fill CSV.to :CTV CBV.to :waste2 # start waste2 flush CS.empty CSV.to :flush CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty # end waste2 flush # SSV already flushed previously CSV.to :flush CS.fill CSV.to :CTV CBV.to :DVB # start DVB flush (7) CS.empty CSV.to :flush CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty # end DBV flush CSV.to :flush CS.fill CSV.to :CTV CBV.to :waste2b # start waste2b flush CS.empty CSV.to :flush CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty CSV.to :air CS.fill CSV.to :CTV CS.empty # end waste2b flush #step 5 Flush PSR PSV.to :PSR PSR.to :sig1 # valve 0 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :sig2 # valve 1 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :sig3 # valve 2 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :conjugate # valve 3 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :sub1 # valve 4 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :sub2 # valve 5 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :wash # valve 6 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :flush # valve 7 PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :PSR PSR.to :sig1 # get flush to clear PSR PS.fill PSR.to :air PS.empty PSR.to :sig1 PS.fill PSR.to :air PS.empty PSV.to :air PS.fill PSV.to :PSR PS.empty #step 6 Flush PSV PSV.to :PSR #clear air clean first; PSR.to :sig1 PS.fill PSV.to :air #air not in flush plumbed clean PSV.empty PSV.to :PSR PS.fill PSV.to :air PSV.empty PSV.to :PSR PS.fill PSV.to :air PS.empty PSV.to :PSR PSR.to :air PS.fill PSV.to :air PSV.empty PSV.to :PSR PS.fill PSV.to :air PSV.empty # end air flush PSV.to :PSR #clean waste1 PSR.to :sig1 PS.fill PSV.to :waste1 PSV.empty PSV.to :PSR PS.fill PSV.to :waste1 PS.empty PSV.to :air PS.fill PSV.to :waste1 PS.empty PSV.to :air PS.fill PSV.to :waste1 PS.empty #end waste1 clean PSV.to :PSR #clean waste2 PSR.to :sig1 PS.fill PSV.to :waste2 PSV.empty PSV.to :PSR PS.fill PSV.to :waste2 PS.empty PSV.to :air PS.fill PSV.to :waste2 PS.empty PSV.to :air PS.fill PSV.to :waste2 PS.empty #end waste2 clean loadFlush :CC #clean CBV line through CC load flush puck CC CBV.to :PSV CTV.to air PSV.to :PSR PSR.to :sig1 PS.fill PSV.to :CBV PSV.empty PSV.to :PSR PS.fill PSV.to :CBV PSV.empty PSV.to :PSR PS.fill PSV.to :CBV PSV.empty PSV.to :air PS.fill PSV.to :CBV PSV.empty PSV.to :air PS.fill PSV.to :CBV PSV.empty PSV.to :air PS.fill PSV.to :CBV PSV.empty returnFlush :CC #end CBV line flush loadFlush :PC # clean PML need flush puck in PC PTV.to :mixing PBV.to :waste1 PSV.to :PSR PSR.to :sig1 PS.fill PSV.to :PML PS.empty PSV.to :PSR PS.fill PSV.to :PML PS.empty PSV.to :PSR PS.fill PSV.to :PML PS.empty PSV.to :air PS.fill PSV.to :PML PS.empty PSV.to :air PS.fill PSV.to :PML PS.empty PSV.to :air PS.fill PSV.to :PML PS.empty #end PML flush PTV.to :PRV #start PSV to CBV line flush (PTV port) PRV.connect :CBV PSV.to :PSR PSR.to :sig1 PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty #end PSV to CBV line flush returnFlush :PC #step 7 Manual PBV Flush through PC use absorbtive towel to catch flush coming out of heater cap port PBV.to :PSV PSV.to :PSR PSR.to :sig1 PS.fill PSV.to :PBV PS.empty PSV.to :PSR PS.fill PSV.to :PBV PS.empty PSV.to :air PS.fill PSV.to :PBV PS.empty PSV.to :air PS.fill PSV.to :PBV PS.empty #step 8 Flush PRV PTV.to :PRV PRV.to :ab1 #flush ab1 PSV.to :PSR PSR.to :sig1 PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty #end ab1 flush PRV.to :ab2 #start flush ab2 PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty #end ab2 flush PRV.to :waste2 #start waste2 flush PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty # end waste2 flush PRV.to :air # start air clean flush PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :PSR PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to :PBV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty PSV.to :air PS.fill PSV.to PTV PS.empty # end air clean flush loadFlush :CC # start PRV/CBV line flush PRV.connect :CBV CTV.to :air PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty # end PRV/CBV line flush returnFlush :CC #step 9 Flush PBV need to load flush puck in PC loadFlush :PC PTV.to :puck PSV.to :PSR PSR.to :sig1 PS.fill PSV.to :PTV PBV.to :air # flush air PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty # end air flush PBV.to :waste1 # start waste1 flush PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty # end waste1 flush PBV.to :waste2 # start waste2 flush PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :PSR PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty PSV.to :air PS.fill PSV.to :PTV PS.empty # end waste2 flush PSV.to :air # clear PSR PS.fill PSV.to :PSR PSR.to :air PS.empty PSV.to :air PS.fill PSV.to :PSR PS.empty # end clear PSR