# macro for creating sample lysate for sand hybe rxn # in situ ESP Delta unit - new valve manifold # lysis solun. pushed in from top; pressure push/mix # rev 047 Aug 18 2003 # mod to REDUCE puck pressure # NO SET rinse # gives ~1.5ml+ ml lysate # heating time 14 min # run time is ~30min # mod for encapsulated heater pad and 5um/0.45um/ss frit assmbly # mod based on the run done 8/12/03 to eliminate PUCK PRESSURE DURING HEAT # 10/10/03 changed temp cal for Ti puck # 11/4/03 reduced swish vol to 1 ml and reduced backoff # 6/17/04 changed temp cal for new Ti pucks (RW; Johnny) # 6/23/ changed protocol slightly based on 6/17 run # 6/24/ revised based on 6/23 run and 6/24 runs (176001tif) # 6/29/04 revised to lower pressuremacro for creating sample lysate for sand hybe rxn # 7/13/04 revised to give ~250 ml sample and longer delay after lysate recovery # 7/20/04 revised to give ~200ml sample # 7/30 revised to give 4 min preheat # 8/13 revised based on sh1b run + M1 flsh PROGRAM: SH1 SYRINGE PULL 22.5 Sample DELAYSEC 30 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 30 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 40 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 50 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 50 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 60 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 80 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample DELAYSEC 80 VALVEPORT Intake HOME SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME SYRINGE PULL 22.5 Sample # sample= 9 x 22.5ml= ~200ml DELAYSEC 90 SYRINGE PUSH ALL Waste VALVEPORT Exhaust HOME #Done sampling SYRINGEJOG PULL 3 #DEPR. EXAHUST VALVEPORT Waste HOME #ISOLATE WASTE LINE DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 VALVEPORT Syringe HOME #NOW DO SAMPLE SIDE SYRINGEJOG PULL 3 DELAYSEC 5 VALVEPORT Sample HOME # depress complete DELAYSEC 15 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 SYRINGE PULL 1.5 M3Bypass DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste # burp M1 loop DELAYSEC 2 SYRINGE PULL 4 M3Bypass VALVEPORT M3Bypass HOME SYRINGEJOG PUSH 3 # INC PRESSURE IN PUCK SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 VALVEPORT Syringe HOME SYRINGEJOG PULL 5 DELAYSEC 10 SYRINGE PULL 5 Air # PUCK DRY OUT COMPLETE DELAYSEC 20 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 7 SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 SYRINGE PULL 1.5 M3Bypass DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste # burp M1 loop DELAYSEC 2 VALVEPORT ToxicWaste HOME SYRINGE PULL 2 FlushM2 DELAYSEC 10 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 DELAYSEC 5 SYRINGE PUSH 5 M1Bypass # CLEAR M1 OF SW DELAYSEC 5 SYRINGEJOG PULL 3 DELAYSEC 5 SYRINGEJOG PULL 3 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 SYRINGE PULL 2 FlushM1 DELAYSEC 7 VALVEPORT FlushM1 HOME SYRINGEJOG PULL 3 DELAYSEC 4 SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 SYRINGE PULL 1.0 Lysis # premix lysis/sample buffer DELAYSEC 5 VALVEPORT Lysis HOME SYRINGEJOG PULL 3.5 # rinse syringe SYRINGE PUSH ALL ToxicWaste DELAYSEC 2 SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop HEAT 46 15 # 6/18/04 cal VALVEPORT Puck SET # relieve vacuum/vent lower loop DELAYSEC 2 VALVEPORT Air SET # vent puck during preheat DELAYMIN 4 SYRINGE PULL 2 Lysis DELAYSEC 5 VALVEPORT Lysis HOME SYRINGEJOG PULL 3.5 # syringe @ 5.5 DELAYSEC 2 SYRINGE PUSH 1.5 Puck DELAYSEC 2 VALVEPORT Puck HOME # leave syringe SET; others home SYRINGEJOG PUSH 3.5 # leaves 0.5mL in syringe DELAYSEC 7 SYRINGEJOG PULL 2 # syringe at 2.5 DELAYMIN 2 SYRINGEJOG PUSH 0.75 SYRINGEJOG PULL 0.75 # SWISh DELAYMIN 2 SYRINGEJOG PUSH 0.75 SYRINGEJOG PULL 0.75 # SWISH DELAYMIN 2 # heater power begins cycling SYRINGEJOG PUSH 0.75 SYRINGEJOG PULL 0.75 # SWISH DELAYMIN 2 SYRINGEJOG PUSH 0.75 SYRINGEJOG PULL 0.75 # SWISH DELAYMIN 2 #SYRINGEJOG PUSH 0.75 #SYRINGEJOG PULL 0.75 # SWISH; syringe @ 2.75 #DELAYMIN 2 # heat turns off w/~1 min left SYRINGEJOG PUSH 1.5 # Syringe @1 SYRINGE PUSH ALL ToxicWaste DELAYSEC 3 VALVEPORT M1Bypass HOME VALVEPORT Syringe HOME SYRINGEJOG PULL 5.5 # draw vacuum; AT 5.5 DELAYSEC 20 SYRINGE PULL 1.5 Air # suck lysate in syringe; AT 7 DELAYSEC 20 VALVEPORT M3Bypass SET # syringe valve now SET DELAYSEC 5 VALVEPORT M3Bypass HOME # isolate lysate; syringe and air SET END