# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push/mix
# rev 048 Aug 21 2003
# mod to REDUCE puck pressure
# NO SET rinse
# gives ~1.5ml+ ml lysate
# heating time 16 min
# run time is ~26min
# mod for encapsulated heater pad and 5um/0.45um/ss frit assmbly
# mod based on the run done 8/12/03 to eliminate PUCK PRESSURE DURING HEAT
# mod for 0.2 filter
# mod to inc pre-heat time by 1 min 8/21/03
# mod for Ti puck heat temp changed to 42.5
# mod lysis solun push/pulls for frozen filters 10/30/03
# mod 7/29/03 for new syringe
# mod 7/30 for longer preheat
# mod 8/11/04 for sample under pressure
# mod 8/12 to flush M1 to prevent sw contam
# mod 8/13 based on 8/12 run notes
PROGRAM: SH1B
SYRINGE PULL 10 Sample	# prime puck
DELAYSEC 20
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 30
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample	# sample vol ~50ml
DELAYSEC 45
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME		#Done sampling
SYRINGEJOG PULL 3		#DEPR. EXAHUST 
VALVEPORT Waste HOME		#ISOLATE WASTE LINE
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
VALVEPORT Syringe HOME		#NOW DO SAMPLE SIDE
SYRINGEJOG PULL 3
DELAYSEC 5
VALVEPORT Sample HOME		# depress complete
DELAYSEC 15
VALVEPORT M3Bypass SET
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME	
SYRINGEJOG PULL 10
DELAYSEC 45
SYRINGE PULL 7 Air
DELAYSEC 45
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.5 M3Bypass
DELAYSEC 2
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 2
VALVEPORT ToxicWaste HOME
SYRINGE PULL 2 FlushM2
DELAYSEC 10
VALVEPORT FlushM2 HOME
SYRINGEJOG PULL 4
DELAYSEC 5
SYRINGE PUSH 5 M1Bypass		# CLEAR M1 OF SW
DELAYSEC 5
SYRINGEJOG PULL 3
DELAYSEC 5
SYRINGEJOG PULL 3
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 2 FlushM1
DELAYSEC 7
VALVEPORT FlushM1 HOME
SYRINGEJOG PULL 3
DELAYSEC 4
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
DELAYSEC 2
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
HEAT 46 15			# 6/18/04 cal
VALVEPORT Puck SET		# relieve vacuum/vent lower loop
DELAYSEC 2
VALVEPORT Air SET		# vent puck during preheat
DELAYMIN 4
SYRINGE PULL 2 Lysis
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# syringe @ 5.5
DELAYSEC 2
SYRINGE PUSH 1.5 Puck
DELAYSEC 2
VALVEPORT Puck HOME		# leave syringe SET; others home
SYRINGEJOG PUSH 3.5		# leaves 0.5mL in syringe
DELAYSEC 7
SYRINGEJOG PULL 2		# syringe at 2.5
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISh
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH
DELAYMIN 2			# heater power begins cycling
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH
DELAYMIN 2
#SYRINGEJOG PUSH 0.75
#SYRINGEJOG PULL 0.75		# SWISH; syringe @ 2.75
#DELAYMIN 2			# heat turns off w/~1 min left
SYRINGEJOG PUSH 1.5		# Syringe @1
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 3
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5.5		# draw vacuum; AT 5.5
DELAYSEC 10
SYRINGE PULL 1.5 Air		# suck lysate in syringe; AT 7
DELAYSEC 10
VALVEPORT M3Bypass SET		# syringe valve now SET
DELAYSEC 5
VALVEPORT M3Bypass HOME		# isolate lysate; syringe and air SET
END