# Array dev- part 2 of the filter Sand Hybe process # ESP DELTA unit with new valve manifold # rev June 18 2004 # Edited from 46j with 10 sec delay after sub addition # Edited from 46j with altered pressure pushes for new heater pads # Edited from 46m with alternative protocol for adding fluid and swish # Starts with ~1.5ml lysate from sh1 # Syringe begins @ pos 7 mL # valve config 001 # camera exposures 120 sec with bin = 2 # added delays to help with fluid flow, etc # all fluid flow is from top to bottom with pressure push for mixing # 12 min hybe, 8 min sig, 5 min con # all washes are 2 min # filter top back-off to 0.673 # use 24mm die for cutting arrays! # addition of sub as per sh2_45b - 1ml with dry-out # compares to sh2_45h except last wash step is mod to prevent + pressure # also added flush of M2 post image to clean out sub # vol of wash/sig/con is increased to deliver 2ml to puck # vols adj based on sh2_46g +cont run 5/16 # added 4th wash post con # NO lysis kill step # push/pull pressure swish vol for wash, sig, con at 0.5 # for HAB arrays - calls out sig 1 # 8/22/03 added EtOH/SET rinse to syringe pre sub/M2 flsh to kill con # 10/10/03 changed temp setting to 34.5 for Ti puck # 06/17/04 changed temp for new CPEEK; backoff = 0.673 final # starts with Syringe and Air SET # differs from SH2A in clearing of sub from puck (controlled) PROGRAM: SH2AA HEAT 35.5 91 # CPEEK, 30C; modify time for real SH2 DELAYMIN 2 VALVEPORT Air HOME SYRINGEJOG PUSH 6 DELAYMIN 4 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 3 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 3 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 4 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 3 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 3 # hybe t=~20 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Wash SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end sample Wash; 2 min DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for sig SYRINGE PULL 2 Signal1 DELAYSEC 5 VALVEPORT Signal1 HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 5 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 2 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 2 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 2 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 2 # sig probe hybe complete t= ~8 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Wash SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end Sig Wash 1; 2 min DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Sig Wash2 SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end Sig Wash2; 2 min DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Con SYRINGE PULL 2 Conjugate DELAYSEC 5 VALVEPORT Conjugate HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 5 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 2 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 2 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 2 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 # con complete t= ~7 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Con Wash1 SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end Con Wash1; 2 min DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Con Wash2 SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end sample Con Wash2; 2 min DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Con Wash3 SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end sample Con Wash3; 2 min DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Con Wash4 SYRINGE PULL 2 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 5 # clear mnfld DELAYSEC 2 VALVEPORT M1Bypass HOME SYRINGEJOG PUSH 6 DELAYMIN 1 # LEAVES SYRINGE AT 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 DELAYMIN 1 SYRINGEJOG PULL 0.5 DELAYSEC 5 # move fluid by pressure release/push cycle SYRINGEJOG PUSH 0.5 # end sample Con Wash4; 2 min CAMERAPOWER ON # START CAMERA COOLING DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 7 # draw slight vacuum SYRINGE PULL 3 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 3 VALVEPORT Air HOME SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for syringe wash/sub SYRINGE PULL 2.5 Fixative # try to kill residual con with EtOH/SET; now 70% EtOH DELAYSEC 5 VALVEPORT Fixative HOME SYRINGEJOG PULL 4 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1.5 M2Bypass # pull in air to blow remaining fluid SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 3.5 FlushM2 DELAYSEC 10 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 DELAYSEC 7 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 2 M2Bypass # pull in air to blow remaining fluid SYRINGE PUSH ALL ToxicWaste # flushed; ready for sub SYRINGE PULL 1.0 Sub1 DELAYSEC 5 SYRINGE PULL 1.0 Sub2 DELAYSEC 5 VALVEPORT Sub2 HOME SYRINGEJOG PULL 5 # mix sub 1&2 VALVEPORT M2Bypass HOME SYRINGEJOG PUSH 6 DELAYSEC 7 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 6.5 # draw slight vacuum SYRINGE PULL 1 Air # air out filter# air out puck for imaging ACQUIREIMAGE LIGHT, 2X2, 120000, "120 sec b2" CAMERAPOWER OFF #SHUT CAMERA DOWN SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 3 FlushM2 DELAYSEC 7 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1.5 M2Bypass # pull in air to blow remaining fluid SYRINGE PUSH ALL ToxicWaste END