# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push/mix
# rev 045 Aug 12 2003
# mod to reduce puck pressure
# NO SET rinse
# no 2nd air-out post sample
# gives ~1.5ml+ ml lysate
# heating time 15 min
# run time is ~26min
# mod for encapsulated heater pad and 5um/0.45um/ss frit assmbly
# mod based on the run done 8/4/03 with sh1s_2.txt
# mod based on the run done 8/12/03 to eliminate sw backflow thru TWaste
PROGRAM: SSH1
SYRINGE PULL 5 Sample		# prime puck
DELAYSEC 20
SYRINGE PUSH ALL ToxicWaste		# should be Waste for in situ
SYRINGE PULL 22.5 Sample
DELAYSEC 30                    #  increased to compensate for 0.45 sandwich
VALVEPORT Sample HOME
SYRINGE PUSH ALL ToxicWaste    #  Changed to prevent SW contamination
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5
DELAYSEC 20
SYRINGE PULL 3.0 Air
DELAYSEC 30
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.5 M3Bypass
DELAYSEC 2
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 2
SYRINGE PULL 4 M3Bypass
VALVEPORT M3Bypass HOME
SYRINGEJOG PUSH 3		# INC PRESSURE IN PUCK
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5
DELAYSEC 10
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PULL 5 Air
DELAYSEC 20
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 5
SYRINGE PULL 2.2 Lysis		# incl comp for backlash
DELAYSEC 7                     	# increase from 5 to 7 sec
VALVEPORT Lysis HOME
SYRINGEJOG PULL 4.25		# should leave 2ml in syringe
SYRINGE PUSH 2.5 Puck		# prime top loop
DELAYSEC 3
VALVEPORT Puck HOME		# isolate puck
SYRINGEJOG PUSH 3		# leaves 0.95mL in syringe
DELAYSEC 5
SYRINGEJOG PULL 2.5		# RELIEVE PRESSURE; syringe
HEAT 57 15			# 85C
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 2
SYRINGEJOG PUSH 1.5
SYRINGEJOG PULL 1.5		# PRESSURE PUSH
DELAYMIN 1			# heat ends @15min
SYRINGEJOG PUSH 2.5		# inc puck pressure; syringe @ 0.95
SYRINGE PUSH 0.7 ToxicWaste	# blow off SOME pressure; syringe @ 0.25
DELAYSEC 3
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4.5		# draw vacuum; AT 4.75
DELAYSEC 10
SYRINGE PULL 2.25 Air		# suck lysate in syringe; AT 7
DELAYSEC 15
VALVEPORT M3Bypass SET
DELAYSEC 2
VALVEPORT Syringe SET		# isolate lysate in syringe
END