# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push/mix
# rev 047 Aug 18 2003
# mod to REDUCE puck pressure
# NO SET rinse
# gives ~1.5ml+ ml lysate
# heating time 14 min
# run time is ~30min
# mod for encapsulated heater pad and 5um/0.45um/ss frit assmbly
# mod based on the run done 8/12/03 to eliminate PUCK PRESSURE DURING HEAT
# 10/10/03 changed temp cal for Ti puck
# 11/4/03 reduced swish vol to 1 ml and reduced backoff
# 6/17/04 changed temp cal for new Ti pucks (RW; Johnny)
# 6/23/ changed protocol slightly based on 6/17 run
# 6/24/ revised based on 6/23 run and 6/24 runs (176001tif)
# 6/29/04 revised to lower pressure
# 7/14/04 revised to pull sample through intake loop without preesure pot
PROGRAM: SSH1_PIN
SYRINGE PULL 22.5 Sample		# prime intake loop and puck
DELAYSEC 20
SYRINGE PUSH ALL ToxicWaste		# should be Waste for in situ
SYRINGE PULL 22.5 Sample
DELAYSEC 30                    #  increased to compensate for 0.45 sandwich
VALVEPORT Sample HOME
SYRINGE PUSH ALL ToxicWaste    #  Changed to prevent SW contamination
SYRINGE PULL 22.5 Sample       #  finish clearing intake loop
DELAYSEC 30                    #  increased to compensate for 0.45 sandwich
VALVEPORT Sample HOME
SYRINGE PUSH ALL ToxicWaste    #  Changed to prevent SW contamination
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5
DELAYSEC 20
SYRINGE PULL 3.0 Air
DELAYSEC 20
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.5 M3Bypass
DELAYSEC 2
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 2
SYRINGE PULL 4 M3Bypass
VALVEPORT M3Bypass HOME
SYRINGEJOG PUSH 3		# INC PRESSURE IN PUCK
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5
DELAYSEC 10
SYRINGE PULL 5 Air
DELAYSEC 20
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.5 M3Bypass
DELAYSEC 2
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 2
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
HEAT 46 15			# 6/18/04 cal
VALVEPORT Puck SET		# relieve vacuum/vent lower loop
DELAYSEC 2
VALVEPORT Air SET		# vent puck during preheat
DELAYMIN 2
SYRINGE PULL 2 Lysis
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# syringe @ 5.5
DELAYSEC 2
SYRINGE PUSH 1.5 Puck
DELAYSEC 2
VALVEPORT Puck HOME		# leave syringe SET; others home
SYRINGEJOG PUSH 3.5		# leaves 0.5mL in syringe
DELAYSEC 7
SYRINGEJOG PULL 2		# syringe at 2.5
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISh
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH
DELAYMIN 2			# heater power begins cycling
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH
DELAYMIN 2
SYRINGEJOG PUSH 0.75
SYRINGEJOG PULL 0.75		# SWISH; syringe @ 2.75
DELAYMIN 2			# heat turns off w/~1 min left
SYRINGEJOG PUSH 1.5		# Syringe @1
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 3
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5.5		# draw vacuum; AT 5.5
DELAYSEC 10
SYRINGE PULL 1.5 Air		# suck lysate in syringe; AT 7
DELAYSEC 10
VALVEPORT M3Bypass SET		# syringe valve now SET
DELAYSEC 5
VALVEPORT M3Bypass HOME		# isolate lysate; syringe and air SET
END