# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top
# rev 34 Dec 20, 2002
# short volume for culture test modified from 10/3/02 deployment 
# the 5xSET 0.01% IGEPAL rinse is included
# ~2 ml lysate with PRESSURE PUSH during heating
# heating time 10; 9 min with sample
# PUCK HITS RIGHT TEMP IN ~8 MIN - CAL IS OFF
# NO SWISH
PROGRAM: SH1
SYRINGE PULL 5 Sample		# prime puck
DELAYSEC 10
SYRINGE PUSH ALL Waste
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME          #Done sampling
SYRINGE PULL 4.0 SETrinse 	# 5x SET with 0.01% IGEPAL
DELAYSEC 5
VALVEPORT SETrinse HOME
SYRINGEJOG PULL 3		# clear manifold
SYRINGE PUSH 6 Puck		# clear syringe block mnfld
DELAYSEC 10
VALVEPORT Puck HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 7
DELAYSEC 10
SYRINGE PULL 3.0 Air
DELAYSEC 10
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 5 Air		# 2nd draw of air to dry out puck
DELAYSEC 10
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
SYRINGE PULL 2.25 Lysis
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 1.75	# clear mnfld
HEAT 62.4 10		# check time
DELAYSEC 2
SYRINGE PUSH 1.25 Puck
VALVEPORT Puck HOME
SYRINGEJOG PUSH ALL
DELAYMIN 3
SYRINGEJOG PULL 0.5
SYRINGEJOG PUSH ALL
DELAYMIN 3
SYRINGEJOG PULL 0.5
SYRINGEJOG PUSH ALL
DELAYMIN 4		# cook t= 10 min
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5	# syringe @ 5ml
DELAYSEC 10
VALVEPORT Syringe SET		# ISOLATGE LYSATE IN SYRINGE
VALVEPORT M1Bypass SET		# RELIEVE VACUUM/PRESSURE
DELAYSEC 2
SYRINGE PUSH 1 Puck		# recycle lysate 2nd pass; syringe @ 4
VALVEPORT Puck HOME
SYRINGEJOG PUSH 3.5		# LEAVES SYRINGE AT 0.5ml
DELAYSEC 10
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4.5		# draw vacuum; SYRNGE @5
DELAYSEC 5
VALVEPORT Syringe SET		# ISOLATGE LYSATE IN SYRINGE
VALVEPORT M3Bypass SET		# RELIEVE VACUUM
DELAYSEC 2
SYRINGE PULL 2 Air		# suck in lysate; syringe @7 ml
DELAYSEC 2O
VALVEPORT M3Bypass SET		# to relieve vacuum in syringe
DELAYSEC 3
VALVEPORT M3Bypass HOME
VALVEPORT Air SET
END