# Array dev- part 2 of the filter Sand Hybe process # ESP DELTA unit with new valve manifold # rev 010; July 17 2002 # Edited from GOM macro # Starts with ~1.5 ml lysate from sandhybe1 macro # Syringe begins @ pos 5 mL # valve config 001 # no delays between steps now reduce 10 minutes # clear filter between washes with syringe pull # increased push pulls signal/conj fills # reduced con wash preeure strokes # incl. Lysis syringe rinse to kill conj B/4 add sub PROGRAM: SH2a SYRINGE PULL 0.75 Air SYRINGEJOG PUSH ALL DELAYMIN 2 # con complete t=8min SYRINGE PULL 3.5 Air # air out puck SYRINGE PUSH ALL ToxicWaste # puck cleared SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for con wash1 SYRINGE PULL 2 Wash # Vector Buffer E VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld SYRINGE PUSH 1.5 Puck # prime connection to puck VALVEPORT Puck HOME SYRINGEJOG PUSH 1.7 SYRINGEJOG PULL 1.7 # relieve pressure VALVEPORT Puck SET # prime lower loop VALVEPORT Puck HOME # puck filled with wash fluid SYRINGE PUSH ALL ToxicWaste DELAYSEC 30 SYRINGE PULL 0.75 Air SYRINGEJOG PUSH ALL # array bkflsh DELAYSEC 30 # end con wash 1 SYRINGE PULL 4 Air # air out puck SYRINGE PUSH ALL ToxicWaste # puck cleared SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for con wash 2 SYRINGE PULL 2 Wash # Vector Buffer E VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld SYRINGE PUSH 1.5 Puck # prime connection to puck VALVEPORT Puck HOME SYRINGEJOG PUSH 1.7 SYRINGEJOG PULL 1.7 # relieve pressure VALVEPORT Puck SET # prime lower loop VALVEPORT Puck HOME # puck filled with wash fluid SYRINGE PUSH ALL ToxicWaste DELAYSEC 30 SYRINGE PULL 0.75 Air SYRINGEJOG PUSH ALL # array bkflsh DELAYSEC 30 # end con wash 2 SYRINGE PULL 4 Air # air out puck SYRINGE PUSH ALL ToxicWaste # puck cleared SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for con wash 3 SYRINGE PULL 2 Wash # Vector Buffer E VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld SYRINGE PUSH 1.5 Puck # prime connection to puck VALVEPORT Puck HOME SYRINGEJOG PUSH 1.7 SYRINGEJOG PULL 1.7 # relieve pressure VALVEPORT Puck SET # prime lower loop VALVEPORT Puck HOME # puck filled with wash fluid SYRINGE PUSH ALL ToxicWaste DELAYSEC 30 SYRINGE PULL 0.75 Air SYRINGEJOG PUSH ALL # array bkflsh DELAYSEC 30 # end con wash 3 SYRINGE PULL 4 Air # air out puck SYRINGE PUSH ALL ToxicWaste # puck cleared SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Kill step SYRINGE PULL 2 Lysis # KILL CON IN SYRINGE DELAYSEC 10 VALVEPORT Lysis HOME SYRINGEJOG PULL 3 DELAYSEC 10 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 5 FlushM2 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 # clears out manifold SYRINGE PUSH ALL ToxicWaste # ends lysis poison SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop CAMERAPOWER ON # START CAMERA COOLING SYRINGE PULL 4 FlushM2 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1.5 M2Bypass # pull in air to blow remaining fluid SYRINGE PUSH ALL ToxicWaste # flushed; ready for sub SYRINGE PULL 1.0 Sub1 SYRINGE PULL 1.0 Sub2 VALVEPORT Sub2 HOME SYRINGEJOG PULL 4 # mix sub 1&2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 3.5 # CK VOL TO MAKE SURE ALL IS IN PUCK! DELAYSEC 10 SYRINGE PUSH ALL Puck SYRINGE PULL 4 Air # air out puck for imaging ACQUIREIMAGE LIGHT, 2X2, 60000, "1 min" ACQUIREIMAGE LIGHT, 2X2, 240000, "3 min" CAMERAPOWER OFF #SHUT CAMERA DOWN SYRINGE PUSH ALL ToxicWaste END