# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push/mix
# rev 048 Aug 21 2003
# mod to REDUCE puck pressure
# NO SET rinse
# gives ~1.5ml+ ml lysate
# heating time 16 min
# run time is ~26min
# mod for encapsulated heater pad and 5um/0.45um/ss frit assmbly
# mod based on the run done 8/12/03 to eliminate PUCK PRESSURE DURING HEAT
# mod for 0.2 filter
# mod to inc pre-heat time by 1 min 8/21/03
# mod for Ti puck heat temp changed to 42.5
# mod lysis solun push/pulls for frozen filters 10/30/03
# mod 7/29/03 for new syringe
# mod 7/30 for longer preheat
# mod 8/11/04 for sample under pressure
# THIS FOR TESTING SAMPLE ACQUISITION UNDER PRESSURE ONLY
PROGRAM: SH1TST
SYRINGE PULL 7.5 Sample	# prime puck
DELAYSEC 20
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 30
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample	# sample vol ~50ml
DELAYSEC 45
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME		#Done sampling
SYRINGEJOG PULL 2		#DEPR. EXAHUST 
VALVEPORT Waste HOME		#ISOLATE WASTE LINE
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
VALVEPORT Syringe HOME		#NOW DO SAMPLE SIDE
SYRINGEJOG PULL 2
DELAYSEC 5
VALVEPORT Sample HOME		# depress complete
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME	
SYRINGEJOG PULL 7
DELAYSEC 30
SYRINGE PULL 5.0 Air
DELAYSEC 30
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PUSH ALL ToxicWaste
DELAYSEC 2
SYRINGE PULL 1.5 M3Bypass
DELAYSEC 2
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
END