# lysate filtration used 6-9-03
# rev 007; April 21 2003; minor mod 4/22 based on run notes
# Starts with ~2ml 5um lysate from sh1_37 macro
# passes lysate from SH1 through 0.22um Durapore with ss screen backing 1X
# Syringe begins @ pos 7 mL
# valve config 001
# includes syringe rinse step with lysis buffer
# yields ~0.5ml< than input for SH2
# #'d steps could be implemented for rinsing upper loop or dil lysate
# takes ~3.5 min to run
PROGRAM: LYFIL
HEAT 37 3		# 30C; ck time
SYRINGE PUSH 1.5 Puck
VALVEPORT Puck HOME
SYRINGEJOG PUSH 4.5	#LEAVES SYRINGE AT 0.5ml
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp loop
SYRINGE PULL 1 Lysis
DELAYSEC 2
VALVEPORT Lysis HOME
SYRINGEJOG PULL 2
SYRINGE PUSH ALL ToxicWaste	# blow out particles in syringe
SYRINGE PULL 1 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp loop; syringe clean
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 5		# may want to do this in small steps?
DELAYSEC 30
SYRINGE PULL 2 Air		# syringe at 7
DELAYSEC 30
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 3
VALVEPORT M3Bypass HOME
VALVEPORT Syringe SET		# leaves lysate trapped
END