# Array dev- part 2 of the filter Sand Hybe process used 6-9-03 # ESP DELTA unit with new valve manifold # rev 046i; May 26 2003 # Edited from Oct '02 deployment # Starts with ~1.5ml lysate from sh1_036/lyfil_007 macros # Syringe begins @ pos 7 mL # valve config 001 # camera exposures 120 sec with bin = 2 # added delays to help with fluid flow, etc # all fluid flow is from top to bottom with pressure push for mixing # 12 min hybe, 8 min sig, 5 min con # all washes are 2 min # filter top back-off <1/4, >1/8 turn # use 24mm die for cutting arrays! # addition of sub as per sh2_45b - 1ml with dry-out # compares to sh2_45h except last wash step is mod to prevent + pressure # also added flush of M2 post image to clean out sub # vol of wash/sig/con is increased to deliver 2ml to puck # vols adj based on sh2_46g +cont run 5/16 # added 4th wash post con # NO lysis kill step # push/pull pressure swish vol for wash, sig, con at 1.25 # calls sig1 for HAB arrays PROGRAM: SH2A VALVEPORT Air SET # vent puck during pre-heat to avoid +pressure HEAT 37 65 # 37=30C puck; modify time for real SH2 DELAYMIN 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 4.5 #LEAVES SYRINGE AT 1 DELAYSEC 3 SYRINGEJOG PULL 2 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 2 SYRINGEJOG PULL 2 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 2 SYRINGEJOG PULL 2 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 2 SYRINGEJOG PULL 2 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 2 SYRINGEJOG PULL 2 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 2 SYRINGEJOG PULL 2 # move fluid by pressure release/push cycle DELAYMIN 2 # hybe t=~12 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 3 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Wash SYRINGE PULL 2.25 Wash DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 # LEAVES SYRINGE AT 0.25 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end sample Wash; 2 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for SIG SYRINGE PULL 2.25 Signal1 DELAYSEC 5 VALVEPORT Signal1 HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # sig probe hybe complete t= ~8 min SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for SIG wash1 SYRINGE PULL 2.25 Wash # Vector Buffer E + 0.05% Tween 20 DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end sig Wash 1; 2 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for sig wash 2 SYRINGE PULL 2.25 Wash # Vector Buffer E + 0.05% Tween 20 DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end sig Wash 2; 2 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for con SYRINGE PULL 2.25 Conjugate DELAYSEC 5 VALVEPORT Conjugate HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 2 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # con complete t= ~5+ min SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON wash1 SYRINGE PULL 2.25 Wash # Vector Buffer E + 0.05% Tween 20 DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end con Wash 1; 2 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON wash 2 SYRINGE PULL 2.25 Wash # Vector Buffer E + 0.05% Tween 20 DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end con Wash 2; 2 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for con wash 3 SYRINGE PULL 2.25 Wash # Vector Buffer E + 0.05% Tween 20 DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end con Wash 3; 2 min SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for con wash 4 SYRINGE PULL 2.25 Wash # Vector Buffer E + 0.05% Tween 20 DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.5 DELAYSEC 3 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 SYRINGEJOG PUSH 1.25 SYRINGEJOG PULL 1.25 # move fluid by pressure release/push cycle DELAYMIN 1 # end con Wash 4; 2 min CAMERAPOWER ON # START CAMERA COOLING SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 2.25 # draw slight vacuum SYRINGE PULL 2 Air # air out filter DELAYSEC 2 VALVEPORT M3Bypass SET # relieve vacuum DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for syringe wash/sub SYRINGE PULL 3 FlushM2 DELAYSEC 7 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1.5 M2Bypass # pull in air to blow remaining fluid SYRINGE PUSH ALL ToxicWaste # flushed; ready for sub SYRINGE PULL 1.0 Sub1 DELAYSEC 5 SYRINGE PULL 1.0 Sub2 DELAYSEC 5 VALVEPORT Sub2 HOME SYRINGEJOG PULL 4 # mix sub 1&2 SYRINGE PUSH 1.5 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 3.25 # CK VOL TO MAKE SURE ALL IS IN PUCK! DELAYSEC 10 SYRINGE PUSH ALL Puck DELAYSEC 3 SYRINGE PULL 1 Air # air out puck for imaging ACQUIREIMAGE LIGHT, 2X2, 120000, "120 sec b2" CAMERAPOWER OFF #SHUT CAMERA DOWN SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 3 FlushM2 DELAYSEC 7 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1.5 M2Bypass # pull in air to blow remaining fluid SYRINGE PUSH ALL ToxicWaste END