# macro for creating sample lysate for sand hybe rxn used 6-9-03
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push mix
# rev 041 July 30 2003
# mod to reduce puck pressure and overcome filter bubble pt
# the 4xSET rinse is with 0.005% IGEPAL 
# ~2 ml lysate
# heating time 14 min
# run time is ~26min
PROGRAM: SSH1
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Sample HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME          #Done sampling
SYRINGE PULL 4 SETrinse 	# 5x SET with 0.005% IGEPAL
DELAYSEC 5
VALVEPORT SETrinse HOME
SYRINGEJOG PULL 12		# clear manifold
SYRINGE PUSH 15 Puck		# clear syringe block mnfld
DELAYSEC 20
VALVEPORT Puck HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 10
DELAYSEC 20
SYRINGE PULL 6 Air
DELAYSEC 30
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 15 Air		# 2nd draw of air to dry out puck
DELAYSEC 30
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
SYRINGE PULL 2 Lysis		# incl comp for backlash
DELAYSEC 7                    	# increase from 5 to 30 sec
VALVEPORT Lysis HOME
SYRINGEJOG PULL 4.5		# should leave 2ml in syringe
SYRINGE PUSH 2.25 Puck		# prime top loop
VALVEPORT Puck HOME		# isolate puck
SYRINGEJOG PUSH 3.75		# leaves syringe at .5
DELAYSEC 10
SYRINGEJOG PULL 2		#RELIEVE PRESSURE
HEAT 57 14			# 85C
DELAYMIN 2
SYRINGEJOG PUSH 2
DELAYSEC 10
SYRINGEJOG PULL 2		# PRESSURE PUSH
DELAYMIN 3
SYRINGE PUSH 2 ToxicWaste	# blow off SOME pressure
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4		# draw vacuum; AT 4.5
DELAYSEC 10
SYRINGE PULL 2 Air		# suck lysate in syringe; AT 6.5
DELAYSEC 15
VALVEPORT M3Bypass SET
DELAYSEC 2
SYRINGE PUSH 2.25 Puck		# recycle lysate 2nd pass
VALVEPORT Puck HOME
SYRINGEJOG PUSH 3.75		# syringe at .5
DELAYSEC 10
SYRINGEJOG PULL 2
DELAYMIN 3
SYRINGEJOG PUSH 2		# PRESSURE PUSH
DELAYSEC 5
SYRINGEJOG PULL 2
DELAYMIN 3
SYRINGE PUSH 2 ToxicWaste	# blow off SOME pressure
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4		# draw vacuum; AT 4.5
DELAYSEC 10
SYRINGE PULL 2.5 Air		# suck lysate in syringe; AT 7
DELAYSEC 20
VALVEPORT M3Bypass SET
DELAYSEC 2
VALVEPORT Syringe SET		# isolate lysate in syringe
END