# macro for creating sample lysate for sand hybe rxn used 6-9-03
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push mix
# rev 042 Aug 1 2003
# mod to reduce puck pressure and overcome filter bubble pt
# the 5xSET rinse is with 0.005% IGEPAL 
# gives ~1.5ml+ ml lysate
# heating time 14 min
# run time is ~26min
# mod for encapsulated heater pad and 0.45um/10um filter
# mod based on SH1_41 and the run done 8/1/03 with ssh1c.txt
# deleted 2nd air-out post sample
PROGRAM: SSH1
SYRINGE PULL 5 Sample		# prime puck
DELAYSEC 20
SYRINGE PUSH ALL Waste
SYRINGE PULL 22.5 Sample
DELAYSEC 30                    #  increased to compensate for 0.45 sandwich
VALVEPORT Sample HOME
SYRINGE PUSH ALL ToxicWaste    #  Changed to prevent SW contamination
VALVEPORT Exhaust HOME          #Done sampling
SYRINGE PULL 4.0 SETrinse 	# 4x SET with 0.005% IGEPAL
DELAYSEC 5
VALVEPORT SETrinse HOME
SYRINGEJOG PULL 12		# clear manifold
SYRINGE PUSH 15 Puck		# clear syringe block mnfld
DELAYSEC 20
VALVEPORT Puck HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 10
DELAYSEC 10
SYRINGE PULL 6.0 Air
DELAYSEC 20
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 5
SYRINGE PULL 2 Lysis		# incl comp for backlash
DELAYSEC 7                     	# increase from 5 to 7 sec
VALVEPORT Lysis HOME
SYRINGEJOG PULL 4.5		# should leave 2ml in syringe
SYRINGE PUSH 2.5 Puck		# prime top loop
DELAYSEC 2
VALVEPORT Puck HOME		# isolate puck
SYRINGEJOG PUSH 3.5		# leaves 0.5mL in syringe
DELAYSEC 10
SYRINGEJOG PULL 3		# RELIEVE PRESSURE; syringe @3.5
HEAT 57 14			# 85C
DELAYMIN 2
SYRINGEJOG PUSH 2
DELAYSEC 10
SYRINGEJOG PULL 2		# PRESSURE PUSH
DELAYMIN 3
SYRINGE PUSH 3 ToxicWaste	# blow off SOME pressure
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4		# draw vacuum; AT 4.5
DELAYSEC 10
SYRINGE PULL 2 Air		# suck lysate in syringe; AT 6.5
DELAYSEC 15
VALVEPORT M3Bypass SET
DELAYSEC 2
SYRINGE PUSH 2.5 Puck		# recycle lysate 2nd pass
VALVEPORT Puck HOME
SYRINGEJOG PUSH 3.5		# syringe at .5
DELAYSEC 10
SYRINGEJOG PULL 2
DELAYMIN 3
SYRINGEJOG PUSH 2		# PRESSURE PUSH
DELAYSEC 5
SYRINGEJOG PULL 2
DELAYMIN 3
SYRINGE PUSH 2 ToxicWaste	# blow off SOME pressure
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4		# draw vacuum; AT 4.5
DELAYSEC 10
SYRINGE PULL 2.5 Air		# suck lysate in syringe; AT 7
DELAYSEC 20
VALVEPORT M3Bypass SET
DELAYSEC 2
VALVEPORT Syringe SET		# isolate lysate in syringe
END