# macro for creating sample lysate for sand hybe rxn used 6-9-03
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push mix
# rev 042 Aug 1 2003
# mod to reduce puck pressure and overcome filter bubble pt
# the 5xSET rinse is with 0.005% IGEPAL 
# gives ~1.5ml+ ml lysate
# heating time 14 min
# run time is ~26min
# mod for encapsulated heater pad and 0.45um/10um filter
# mod based on SH1_41 and the run done 8/1/03 with ssh1c.txt
# deleted 2nd air-out post sample
PROGRAM: SSH1
SYRINGE PULL 20 Sample		# prime puck
DELAYSEC 30
SYRINGE PUSH ALL ToxicWaste    #  Changed to prevent SW contamination
VALVEPORT Exhaust HOME          #Done sampling
SYRINGE PULL 4.0 SETrinse 	# 4x SET with 0.005% IGEPAL
DELAYSEC 5
VALVEPORT SETrinse HOME
SYRINGEJOG PULL 12		# clear manifold
SYRINGE PUSH 15 Puck		# clear syringe block mnfld
DELAYSEC 20
VALVEPORT Puck HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 10
DELAYSEC 10
SYRINGE PULL 6.0 Air
DELAYSEC 20
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 5
SYRINGE PULL 2.2 Lysis		# incl comp for backlash
DELAYSEC 7                     	# increase from 5 to 7 sec
VALVEPORT Lysis HOME
SYRINGEJOG PULL 4.3		# should leave 2ml in syringe
SYRINGE PUSH 2.5 Puck		# prime top loop
DELAYSEC 2
VALVEPORT Puck HOME		# isolate puck
SYRINGEJOG PUSH 3.25		# leaves 0.75mL in syringe
DELAYSEC 10
SYRINGEJOG PULL 3		# RELIEVE PRESSURE; syringe @3.5
END