# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top; pressure push mix
# rev 041 July 30
# the 5xSET rinse is with 0.005% IGEPAL 
# ~2 ml lysate
# heating time 14 min
# run time is ~26min
# MOD FOR JUST COLLECTINMG SAMPLE
PROGRAM: SH1S
SYRINGE PULL 22.5 Sample
DELAYSEC 30
VALVEPORT Sample HOME
SYRINGE PUSH ALL ToxicWaste
#VALVEPORT Exhaust HOME          #Done sampling/DEPRESS
VALVEPORT M1Bypass HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 15
DELAYSEC 20
SYRINGE PULL 6.0 Air
DELAYSEC 20
VALVEPORT M3Bypass SET		# relieve vacuum
DELAYSEC 7
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
DELAYSEC 5
SYRINGE PULL 2.2 Lysis		# incl comp for backlash
DELAYSEC 7                     	# increase from 5 to 7 sec
VALVEPORT Lysis HOME
SYRINGEJOG PULL 4.3		# should leave 2ml in syringe
SYRINGE PUSH 2.5 Puck		# prime top loop
DELAYSEC 2
VALVEPORT Puck HOME		# isolate puck
SYRINGEJOG PUSH 3.25		# leaves 0.75mL in syringe
DELAYSEC 10
SYRINGEJOG PULL 3		# RELIEVE PRESSURE; syringe @3.5
END