#This is the SH1 protocal, includes flush sample port
#Chris, Roman, Scott 4/29
PROGRAM: SH1
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10 
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust CLOSE
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste       #done sampling decompress
VALVEPORT Exhaust CLOSE
VALVEPORT Toggle CLOSE
SYRINGEJOG PULL 2.5
DELAYSEC 10
VALVEPORT Sample CLOSE
DELAYSEC 10
VALVEPORT Toggle OPEN
SYRINGEJOG PULL 2
SYRINGE PUSH ALL ToxicWaste  #decompressed begin processing
SYRINGE PULL 5.0 Setrinse    # 5x SET with 0.01% IGEPAL 
DELAYSEC 15
SYRINGE PULL 5.0 AirUpper
DELAYSEC 30
SYRINGE PUSH ALL ToxicWaste #dried out filter
FILTERCLAMP OPEN
SHUTTLE FLUSH       		# begin flush sequence
FILTERCLAMP CLOSE 231000	# for steel flush puck
SYRINGE PULL 22.5 FlushLower
DELAYSEC 5
SYRINGE PUSH ALL Sample
VALVEPORT Intake CLOSE          #BEGIN DECOMP STEP
SYRINGEJOG PULL 2.5
VALVEPORT Sample CLOSE
SYRINGEJOG PULL 5
VALVEPORT Toggle OPEN
SYRINGEJOG PULL 2.5
SYRINGE PUSH ALL ToxicWaste
FILTERCLAMP OPEN
SHUTTLE PROCESS
FILTERCLAMP CLOSE
SYRINGE PULL 2.5 AirUpper 
DELAYSEC 10
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.25 Lysis	# Lysis:Sample Buffer (2:3)
SYRINGE PULL 1.5 AirLower
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 Air
VALVEPORT Air CLOSE
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 2.0 Air
VALVEPORT Air CLOSE
VALVEPORT AirUpper #lower loop vacuum relaeased
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.7 Lysis	# comp for reagent bag vacuum
SYRINGE PULL 0.55 AirLower	# ck this should leave 1.5ml in syringe
HEAT 47 14		   	# real temp = ~85 in ~5min
SYRINGE PUSH 0.5 AirUpper
DELAYSEC 5
SYRINGE PUSH 0.5 AirUpper
DELAYSEC 5
SYRINGE PUSH 0.5 AirUpper
DELAYMIN 3
SYRINGEJOG PULL 2.5
DELAYSEC 5
SYRINGEJOG PUSH 1.7
DELAYMIN 3 
SYRINGEJOG PULL 2.0
DELAYSEC 5
SYRINGEJOG PUSH 1.8
DELAYSEC 150
SYRINGEJOG PULL 2.0
DELAYSEC 5
SYRINGEJOG PUSH 1.8
DELAYSEC 150
SYRINGEJOG PULL 3.5	# should leave syringe at 5.55 ml
END