# This is the sh2vx.txt macros used in 102_002 modified to increase wash time
# after the lysate incubation
# will run with shrt1v.txt and qualtest
# Brent 5/1/01 -- increased heat duration from 45 to 105 min
# 
PROGRAM: SH2
HEAT 20.35 105	# puck T=~25C; check t delay=process t
DELAYMIN 1		# pre-warm array puck
SYRINGE PUSH 0.9 AirUpper # pushing in lysate from SH1 incrementally
DELAYSEC 5
SYRINGE PUSH 0.9 AirUpper # total push = 1.7ml - check this and adj.
DELAYMIN 4
SYRINGE PULL 2.O AirUpper
SYRINGE PUSH 1.8 AirUpper
DELAYMIN 4		   # 2nd lysate push
SYRINGE PULL 2.O AirUpper
SYRINGE PUSH 1.8 AirUpper
DELAYMIN 4		   # 3rd lysate push; total hybe t=12 min
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 5.0 SHWash    # assay wash, vol comp for draw down
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 3.5 AirUpper
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 2.5 SHProbe1  # check vol to make sure filter floods
DELAYMIN 2.0		   # 1st sig probe incubation
SYRINGE PULL 0.25 SHProbe1
SYRINGE PULL 0.25 SHProbe1
DELAYMIN 2.0		   # 2nd sig probe push
SYRINGE PULL 0.25 SHProbe1
SYRINGE PULL 0.25 SHProbe1
DELAYMIN 2.0		   # 3rd sig probe push
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 180
SYRINGE PULL 2.5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 90
SYRINGE PULL 2.5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 90
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 3.5 AirUpper
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 2.5 Conjugate # check vol to make sure filter floods
DELAYMIN 2.0		   # 1st con cycle
SYRINGE PULL 0.5 Conjugate # check vol to make sure filter floods
DELAYMIN 3.0		   # 2nd con push
SYRINGE PULL 0.5 Conjugate # check vol to make sure filter floods
DELAYMIN 2.0
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 180
SYRINGE PULL 2.5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 120
SYRINGE PULL 2.5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 120
SYRINGE PULL 2.5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 120
SYRINGE PULL 2.5 SHWash    # assay wash, vol comp for draw down
DELAYSEC 120
CAMERAPOWER ON            # START CAMERA COOLING
HEAT 20.35 1	# turn off heat in one minute
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 3.5 AirUpper
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 2.5 FlushLower
SYRINGE PULL 2.0 AirLower
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 Air
VALVEPORT Air CLOSE
SYRINGE PUSH ALL ToxicWaste  # clear syringe/loop of flush
SYRINGE PULL 1.25 Sub1
SYRINGE PULL 1.25 Sub2
SYRINGE PULL 4.0 AirLower  # mix sub 1&2
SYRINGE PUSH 2.5 AirUpper  # 1st sub push
SYRINGE PULL 2.5 AirUpper
SYRINGE PUSH 2.5 AirUpper  # 2nd sub push
SYRINGE PULL 2.5 AirUpper
ACQUIREIMAGE LIGHT, 2X2, 120000, "SH TEST 4"
ACQUIREIMAGE LIGHT, 2X2, AUTO, "SH TEST 5"
CAMERAPOWER OFF            #SHUT CAMERA DOWN
SYRINGE PUSH ALL ToxicWaste
END