#This is the SH1 protocal, includes flush sample port
#Chris, Roman, Scott 4/29
PROGRAM: SHORT1
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake CLOSE
SYRINGE PUSH ALL Waste       #done sampling decompress
VALVEPORT Exhaust CLOSE
#SYRINGE PULL 5.0 Setrinse    # 5x SET with 0.01% IGEPAL 
#DELAYSEC 15
SYRINGE PULL 5.0 AirUpper
DELAYSEC 30
SYRINGE PUSH ALL ToxicWaste #dried out filter
FILTERCLAMP OPEN
SHUTTLE FLUSH       		# begin flush sequence
FILTERCLAMP CLOSE 231000	# for steel flush puck
SYRINGE PULL 22.5 FlushLower
DELAYSEC 5
SYRINGE PUSH ALL Sample
VALVEPORT Intake CLOSE          #BEGIN DECOMP STEP
SYRINGEJOG PULL 2.5
VALVEPORT Sample CLOSE
SYRINGEJOG PULL 5
VALVEPORT Toggle OPEN
SYRINGEJOG PULL 2.5
SYRINGE PUSH ALL ToxicWaste
FILTERCLAMP OPEN
SHUTTLE PROCESS
FILTERCLAMP CLOSE
SYRINGE PULL 2.5 AirUpper 
DELAYSEC 10
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.25 Lysis	# Lysis:Sample Buffer (2:3)
SYRINGE PULL 1.5 AirLower
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 Air
VALVEPORT Air CLOSE
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 2.0 Air
VALVEPORT Air CLOSE
VALVEPORT AirUpper #lower loop vacuum relaeased
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.7 Lysis	# comp for reagent bag vacuum
SYRINGE PULL 0.55 AirLower	# ck this should leave 1.5ml in syringe
HEAT 47 14		   	# real temp = ~85 in ~5min
SYRINGE PUSH 0.5 AirUpper
DELAYSEC 5
SYRINGE PUSH 0.5 AirUpper
DELAYSEC 5
SYRINGE PUSH 0.5 AirUpper
DELAYMIN 3
SYRINGEJOG PULL 2.5
DELAYSEC 5
SYRINGEJOG PUSH 1.7
DELAYMIN 3 
SYRINGEJOG PULL 2.0
DELAYSEC 5
SYRINGEJOG PUSH 1.8
DELAYSEC 150
SYRINGEJOG PULL 2.0
DELAYSEC 5
SYRINGEJOG PUSH 1.8
DELAYSEC 150
SYRINGEJOG PULL 3.5	# should leave syringe at 5.55 ml
END