# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top
# rev 30 Oct 1, 2002
# modified Sept 30 2002 for full vol sample
# full vol sample= 12 x 22.5ml 
# the 5xSET 0.01% IGEPAL rinse is included
# ~2 ml lysate with swish during heating
# heating time 15 min
PROGRAM: SH1
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 20
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 20
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 30
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 30
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 30
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 45
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 45
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample
DELAYSEC 60
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME
SYRINGE PULL 22.5 Sample	# sample= 12 x 22.5ml= 270ml
DELAYSEC 60
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME		#Done sampling
SYRINGEJOG PULL 3		#DEPR. EXAHUST 
VALVEPORT Waste HOME		#ISOLATE WASTE LINE
DELAYSEC 5
SYRINGE PUSH ALL Waste
VALVEPORT Syringe HOME		#NOW DO SAMPLE SIDE
SYRINGEJOG PULL 3
DELAYSEC 30
VALVEPORT Air SET
SYRINGEJOG PULL 5
DELAYSEC 5
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste	#done depressurizing
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME		#Done sampling
SYRINGE PULL 4.0 SETrinse 	# 5x SET with 0.01% IGEPAL
DELAYSEC 5
VALVEPORT SETrinse HOME
SYRINGEJOG PULL 3		# clear manifold
SYRINGE PUSH 6 Puck		# clear syringe block mnfld
DELAYSEC 10
VALVEPORT Puck HOME
VALVEPORT Syringe HOME
SYRINGEJOG PULL 7
DELAYSEC 10
SYRINGE PULL 3.0 Air
DELAYSEC 10
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 5 Air		# 2nd draw of air to dry out puck
DELAYSEC 10
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
DELAYSEC 5
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
HEAT 62.4 14		   	# 85C; check time
SYRINGE PULL 2.25 Lysis
DELAYSEC 5
VALVEPORT Lysis HOME
SYRINGEJOG PULL 1.75	# clear mnfld
DELAYSEC 2
SYRINGE PUSH 1.25 Puck
VALVEPORT Puck HOME
SYRINGEJOG PUSH ALL
DELAYSEC 15
VALVEPORT Syringe HOME
SYRINGEJOG PULL 0.25
DELAYSEC 3
SYRINGEJOG PULL 0.25
DELAYSEC 3
SYRINGEJOG PULL 0.4
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYMIN 3		# 1st lysis swish
SYRINGEJOG PULL 0.4
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYMIN 3		# 2nd lysis swish
SYRINGEJOG PULL 0.4
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYMIN 3		# 3rd lysis swish
SYRINGEJOG PULL 0.4
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYSEC 3
SYRINGEJOG PUSH 0.2
DELAYMIN 2		# 4th lysis swish cook t= 11 min
SYRINGEJOG PULL 4.5	# syringe @ 5ml
DELAYSEC 10
VALVEPORT Syringe SET		# ISOLATGE LYSATE IN SYRINGE
VALVEPORT M3Bypass SET		# RELIEVE VACUUM
DELAYSEC 2
SYRINGE PUSH 1 Puck		# recycle lysate 2nd pass; syringe @ 4
VALVEPORT Puck HOME
SYRINGEJOG PUSH 3.5		# LEAVES SYRINGE AT 0.5ml
DELAYSEC 10
VALVEPORT Syringe HOME
SYRINGEJOG PULL 4.5		# draw vacuum; SYRNGE @5
DELAYSEC 5
VALVEPORT Syringe SET		# ISOLATGE LYSATE IN SYRINGE
VALVEPORT M3Bypass SET		# RELIEVE VACUUM
DELAYSEC 2
SYRINGE PULL 2 Air		# suck in lysate; syringe @7 ml
DELAYSEC 2O
VALVEPORT M3Bypass SET		# to relieve vacuum in syringe
DELAYSEC 3
VALVEPORT M3Bypass HOME
VALVEPORT Air HOME
END