# macro for creating sample lysate for sand hybe rxn
# in situ ESP Delta unit - new valve manifold
# lysis solun. pushed in from top
# rev 015 July 20, 2002
# modified from GOM macro for concnetrated sample
# includes prime step for sample
# the 5xSET 0.01% IGEPAL rinse is included
# ~1.5 ml lysate
# heating time 14 min no delays between steps
# Modified to load filter make lysate and then push 
# out M3 for collection.
PROGRAM: SH1
LOADFILTER 6
SYRINGE PULL 5 Sample	
DELAYSEC 15
SYRINGE PUSH ALL Waste		# prime puck
SYRINGE PULL 22.5 Sample
DELAYSEC 10
VALVEPORT Intake HOME
SYRINGE PUSH ALL Waste
VALVEPORT Exhaust HOME		#Done sampling
SYRINGEJOG PULL 2		#DEPR. EXAHUST 
VALVEPORT Waste HOME		#ISOLATE WASTE LINE
VALVEPORT Syringe HOME		#NOW DO SAMPLE SIDE
SYRINGEJOG PULL 2
VALVEPORT Air SET	
SYRINGE PUSH ALL ToxicWaste	#done depressurizing
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp fluid in M1 loop
SYRINGE PULL 4.0 SETrinse 	# 5x SET with 0.01% IGEPAL
VALVEPORT SETrinse HOME
SYRINGEJOG PULL 3		# clear manifold
SYRINGE PUSH ALL Puck		# clear syringe block mnfld
DELAYSEC 5
SYRINGE PULL 2 M2Bypass
SYRINGE PULL 5.0 Air
DELAYSEC 5
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 5 Air		# 2nd draw of air to dry out puck
DELAYSEC 5
VALVEPORT M3Bypass SET		# relieve vacuum in syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.0 Lysis		# premix lysis/sample buffer
VALVEPORT Lysis HOME
SYRINGEJOG PULL 3.5		# rinse syringe
SYRINGE PUSH ALL ToxicWaste
SYRINGE PULL 1.5 M3Bypass
SYRINGE PUSH ALL ToxicWaste	# burp M1 loop
HEAT 62.4 14		   	# 85C; check time
SYRINGE PULL 1.7 Lysis		# no comp for backlash
VALVEPORT Lysis HOME
SYRINGEJOG PULL 2		# should leave 1.5ml in syringe
SYRINGE PUSH 1.25 Puck		# prime top loop
VALVEPORT Puck HOME		# isolate puck
SYRINGEJOG PUSH ALL		# puck filled with lysis buffer
SYRINGEJOG PULL 2.45		# relieve pressure
DELAYMIN 3
SYRINGEJOG PUSH 2
SYRINGEJOG PULL 2
DELAYMIN 3
SYRINGEJOG PUSH 2
SYRINGEJOG PULL 2
DELAYMIN 3
SYRINGEJOG PUSH 2
SYRINGEJOG PULL 2
DELAYMIN 2			# lysate cook t= 11min
SYRINGEJOG PUSH ALL
VALVEPORT Syringe HOME
SYRINGEJOG PULL 2.5		# draw vacuum
SYRINGE PULL 2.5 Air		# suck lysate in syringe
DELAYSEC 5
SYRINGE PUSH 1.5 Puck		# recycle lysate 2nd pass
VALVEPORT Puck HOME
SYRINGEJOG PUSH 3
VALVEPORT Syringe HOME
SYRINGEJOG PULL 2.5		# draw vacuum
SYRINGE PULL 4 Air		# suck lysate in syringe; @5 ml
DELAYSEC 5
VALVEPORT M3Bypass SET		# to relieve vacuum in syringe
VALVEPORT Syringe SET		# isolate lysate in syringe
SYRINGEJOG PUSH ALL		#PUSH TO M3 TO COLLECT LYSATE
#VALVEPORT M3Bypass HOME
UNLOADFILTER
END