# Array dev- part 2 of the filter Sand Hybe process FOR SSU # ESP DELTA unit with new valve manifold # rev 040; Sept 10 2002 # Edited from GOM macro # Starts with ~2ml lysate from sh1_27 macro # Syringe begins @ pos 7 mL # valve config 001 # camera exposures 30 sec with bin = 2 # added delays to help with fluid flow, etc # all washes are swish with 1.5 min soak time # uses sig probe equilibration as post-lysate hybe wash PROGRAM: SH2B HEAT 32 72 DELAYMIN 1 SYRINGE PUSH 1 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 5 #LEAVES SYRINGE AT 1 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.4 # prime lower section of filter holder DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 1st lysate swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 2nd lysate swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 3rd lysate push SYRINGEJOG PULL 0.4 DELAYSEC 5 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 4th lysate push SYRINGEJOG PULL 0.2 DELAYSEC 5 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 5th lysate push; hybe t=10 min SYRINGEJOG PULL 4 DELAYSEC 2 SYRINGE PULL 2 Air # clear lysate DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for Sig2 equlibration SYRINGE PULL 2.1 Signal2 DELAYSEC 5 VALVEPORT Signal2 HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 # LEAVES SYRINGE AT 0.25 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 # array bkflsh DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 # end Sig probe equilibration; 2 min SYRINGEJOG PULL 2.25 DELAYSEC 2 SYRINGE PULL 2 Air # clear wash DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for SIG SYRINGE PULL 2.1 Signal2 DELAYSEC 5 VALVEPORT Signal2 HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 1st sig probe swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 2nd sig probe swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 3rd sig probe swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # sig probe hybe complete t= 8min (+2) SYRINGEJOG PULL 2.25 DELAYSEC 3 SYRINGE PULL 2 Air # clear SIG PROBE DELAYSEC 3 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste # puck cleared SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for SIG wash1 SYRINGE PULL 2.1 Wash # Vector Buffer E DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 # end SIG wash1 SYRINGEJOG PULL 2.25 DELAYSEC 3 SYRINGE PULL 2 Air # clear wash DELAYSEC 3 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for SIG wash2 SYRINGE PULL 2.1 Wash # Vector Buffer E DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 # end SIG wash2 SYRINGEJOG PULL 2.25 DELAYSEC 3 SYRINGE PULL 2 Air # clear wash DELAYSEC 3 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON equilibration SYRINGE PULL 2.1 Conjugate DELAYSEC 5 VALVEPORT Conjugate HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 20 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 20 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 20 # end con equilibration; 1 min SYRINGEJOG PULL 2.25 DELAYSEC 2 SYRINGE PULL 2 Air # clear con DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON SYRINGE PULL 2.1 Conjugate DELAYSEC 5 VALVEPORT Conjugate HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 1st con swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 2nd con swish SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYMIN 2 # 3rd con swish; t=6min (+1) SYRINGEJOG PULL 2.25 DELAYSEC 2 SYRINGE PULL 2 Air # clear CON DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste # puck cleared SYRINGE PULL 1.5 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON wash1 SYRINGE PULL 2.1 Wash # Vector Buffer E DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 # end con wash1 SYRINGEJOG PULL 2.25 DELAYSEC 2 SYRINGE PULL 2 Air # clear wash DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON wash2 SYRINGE PULL 2.1 Wash # Vector Buffer E DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 # end con wash2 SYRINGEJOG PULL 2.25 DELAYSEC 2 SYRINGE PULL 2 Air # clear wash DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for CON WASH3 SYRINGE PULL 2.1 Wash # Vector Buffer E DELAYSEC 5 VALVEPORT Wash HOME SYRINGEJOG PULL 2 # clear mnfld DELAYSEC 2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 2.6 DELAYSEC 5 SYRINGE PUSH ALL ToxicWaste VALVEPORT Syringe HOME SYRINGEJOG PULL 0.6 # PRIME LOWER HALF OF PUCK DELAYSEC 3 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 SYRINGEJOG PULL 0.4 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 3 SYRINGEJOG PUSH 0.2 DELAYSEC 40 # end con wash3 SYRINGEJOG PULL 2.25 DELAYSEC 2 SYRINGE PULL 2 Air # clear wash DELAYSEC 2 VALVEPORT M3Bypass SET DELAYSEC 2 SYRINGE PUSH ALL ToxicWaste CAMERAPOWER ON # START CAMERA COOLING SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop;ready for SUB SYRINGE PULL 3 FlushM2 DELAYSEC 5 VALVEPORT FlushM2 HOME SYRINGEJOG PULL 4 # clears out manifold SYRINGE PUSH ALL ToxicWaste SYRINGE PULL 1 M3Bypass SYRINGE PUSH ALL ToxicWaste # burp M1 loop SYRINGE PULL 1.0 Sub1 DELAYSEC 5 SYRINGE PULL 1.0 Sub2 DELAYSEC 5 VALVEPORT Sub2 HOME SYRINGEJOG PULL 4 # mix sub 1&2 SYRINGE PUSH 1.25 Puck VALVEPORT Puck HOME SYRINGEJOG PUSH 4 # leaves syringe at 0.75 DELAYSEC 5 SYRINGEJOG PULL 2 # PRIME LOWER HALF OF PUCK DELAYSEC 5 SYRINGEJOG PUSH 2 DELAYSEC 3 VALVEPORT Syringe HOME SYRINGEJOG PULL 4 # should leave pressure neutral DELAYSEC 3 SYRINGE PULL 3 Air #ACQUIREIMAGE LIGHT, 2X2, 15000, "15 sec b2" ACQUIREIMAGE LIGHT, 2X2, 30000, "30 sec b2" ACQUIREIMAGE LIGHT, 2X2, 60000, "60 sec b2" CAMERAPOWER OFF #SHUT CAMERA DOWN SYRINGE PUSH ALL ToxicWaste END