HR: 15:25h
AN: B52C-06    [PDF]
TI: Mapping Mineral and Microbe Interactions using Laser-based Optical and Chemical Imager (LOCI)
AU: * Scott, J R
EM: scotjr@inel.gov
AF: Idaho National Engineering and Environmental Laboratory (INEEL), 2525 N. Fremont Ave., Idaho Falls, ID 83415 United States
AU: Stoner, D L
EM: dstoner@inel.gov
AF: Idaho National Engineering and Environmental Laboratory (INEEL), 2525 N. Fremont Ave., Idaho Falls, ID 83415 United States
AU: McJunkin, T R
EM: mcjutr@inel.gov
AF: Idaho National Engineering and Environmental Laboratory (INEEL), 2525 N. Fremont Ave., Idaho Falls, ID 83415 United States
AU: Stedtfeld, R D
EM: stedrd@inel.gov
AF: Idaho National Engineering and Environmental Laboratory (INEEL), 2525 N. Fremont Ave., Idaho Falls, ID 83415 United States
AU: Kauffman, M E
EM: kaufme@inel.gov
AF: Idaho National Engineering and Environmental Laboratory (INEEL), 2525 N. Fremont Ave., Idaho Falls, ID 83415 United States
AU: Tremblay, P L
EM: plt@inel.gov
AF: Idaho National Engineering and Environmental Laboratory (INEEL), 2525 N. Fremont Ave., Idaho Falls, ID 83415 United States
AB: Our primary focus has been development of the laser-based optical and chemical imager (LOCI) that provides optical and chemical imaging of complex, heterogeneous sample surfaces. LOCI combines a Fourier transform mass spectrometer (FTMS), unique laser-scanning system, custom optics for surface fluorescence detection, and software for automated data acquisition and analysis. A key feature of LOCI is the unique laser-scanning device that provides high spatial reproducibility true depth-profiling studies and has been automated to provide imaging capabilities. The excellent reproducibility and wavelength independence of the laser-scanning device affords the opportunity to interrogate the same sample surface via optical spectroscopy prior to obtaining mass spectra. Interpretation of mass spectra has been automated using a Fuzzy-logic inference engine, which also produces the surface maps. Our current imaging interests include characterizing heterogeneous minerals, such as basalt, with and without microbe present to determine microbial affinities for specific mineral phases. Additionally, we are interested in characterizing surface contaminant speciation.
DE: 0400 Biogeosciences
SC: Biogeosciences [B]
MN: 2003 Fall Meeting