HR: 0800h
AN: B31A-0958    [Abstracts]
TI: Arsenic Concentrations and Speciation in Shellfishes from Korea
AU: * Yoon, C
EM: chyoon@kbsi.re.kr
AF: Korea Basic Science Institute, Hazardous Substance Research Team, 126-16, 5St., Anam-dong, Sungbuk-ku, Seoul, 136-701 Korea, Republic of
AU: Yoon, H
EM: dunee@kbsi.re.kr
AF: Korea Basic Science Institute, Hazardous Substance Research Team, 126-16, 5St., Anam-dong, Sungbuk-ku, Seoul, 136-701 Korea, Republic of
AB: Speciation of arsenic has received significant attention over the past 20 years in both mechanistic and exposure assessment research. Because the toxicity of arsenic is related to its oxidation state and its chemical forms, the determination of the total arsenic contents in a sample is not adequate to allow its impact on living organisms to be estimated. The inorganic arsenic species, arsenite (As³⁺) and arsenate (As⁵⁺), have been classified as carcinogenic and the methylated forms, monomethyl arsonic acid (MMA) and dimethyl arsinic acid (DMA) have recently been identified as cancer promoters. The highly methylated compounds like as arsenobetaine (AsB) and arsenocholine (AsC) are considered to be nontoxic. Although organisms in marine environment contain high amounts of total arsenic (ppm level), it is not usually present as inorganic arsenic or simple methylated forms well known as one of the toxic species. Arsenobetaine is the dominant species in marine animals and arsenosugars are most abundant in marine algae. This study aims to clarify those arsenic species present in the whole body of eleven different shellfishes from Korea. And those arsenic species were separated and measured by characterization using high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) coupled system. The separation of arsenic species was achieved on anion exchange column and cation exchange column using phosphate and pyridine eluent, respectively. The ultrasonic extraction was employed for extraction of arsenic from whole body of shellfishes. The method was validated by analyzing three certified reference materials (DORM-2, TORT-2, 1566b). Total arsenic concentrations ranged from 0.1 mg/kg dry mass to 21.7 mg/kg dry mass. Most marine shellfishes contained higher arsenobetaine and arsenocholine with the exception of two shellfishes living in river. The lower amounts of inorganic arsenic species were also found in the some sample extracts. Detection of inorganic arsenic can be explained by the conversion of inorganic arsenic to organic arsenic compounds in digestion system in the body may be occurring.
DE: 1000 GEOCHEMISTRY
DE: 1050 Marine geochemistry (4835, 4845, 4850)
DE: 1055 Organic and biogenic geochemistry
SC: Biogeosciences [B]
MN: Fall Meeting 2005