HR: 0800h
AN: B41C-0217    [Abstracts]
TI: Bacteria Community in the Terrestrial Deep Subsurface Microbiology Research of the Chinese Continent Scientific Drilling
AU: Wang, Y
EM: ylwang@mail.im.ac.cn
AF: Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100080 China
AU: Xia, Y
EM: xiay@mail.im.ac.cn
AF: Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100080 China
AU: Dong, H
EM: dongh@muohio.edu
AF: Miami University, Department of Geology, Oxford, OH 45056 United States
AU: Dong, X
EM: dongxz@mail.im.ac.cn
AF: Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100080 China
AU: Yang, K
EM: yangk@mail.im.ac.cn
AF: Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100080 China
AU: * Dong, Z
EM: dongzy@sun.im.ac.cn
AF: Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100080 China
AU: Huang, L
EM: huangl@sun.im.ac.cn
AF: Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100080 China
AB: Microbial communities in the deep drill cores from the Chinese Continent Scientific Drilling were analyzed with culture-independent and dependent techniques. Genomic DNA was extracted from two metamorphic rocks: S1 from 430 and S13 from 1033 meters below the ground surface. The 16S rRNA gene was amplified by polymerase chain reaction (PCR) followed by cloning and sequencing. The total cell number was counted using the 4',6-diamidino-2-phenylindole (DAPI) staining and biomass of two specific bacteria were quantified using real-time PCR. Enrichment was set up for a rock from 3911 meters below the surface in medium for authotrophic methanogens (i.e., CO₂ + H₂). The total cell number in S13 was 1.0 × 10⁴ cells per gram of rock. 16S rRNA gene analysis indicated that low G + C Gram positive sequences were dominant (50 percent of all 54 clone sequenced) followed by the alpha-, beta, and gamma-Proteobacteria. Within the low G + C Gram positive bacteria, most clone sequences were similar to species of Bacillus from various natural environments (deserts, rivers etc.). Within the Proteobacteria, our clone sequences were similar to species of Acinetobacter, Acidovorax, and Aeromonas. The RT-RCP results showed that biomass of two particular clone sequences (CCSD1305, similar to Aeromonas caviae and CCSD1307, similar to Acidovorax facilis) was 95 and 1258 cells/g, respectively. A bacterial isolate was obtained from the 3911-m rock in methanogenic medium. It was Gram negative with no flagella, immobile, and facultative anaerobic, and grows optimally at 65^oC. Phylogenetic analysis indicated that it was closely related to the genus of Bacillus. Physiological tests further revealed that it was a strain of Bacillus caldotenax.
DE: 0400 BIOGEOSCIENCES
SC: Biogeosciences [B]
MN: Fall Meeting 2005