HR: 1340h
AN: B43A-0245    [Abstracts]
TI: Identification of newly grown microbial populations in soil through stable isotope probing with [18O]water.
AU: * Schwartz, E
EM: Egbert.Schwartz@nau.edu
AF: Northern Arizona University, Box 5640 Department of Biological Sciences, Flagstaff, AZ 86011-5640 United States
AB: Soil samples were incubated with [18O]water for 14 days and isopycnic centrifugation of the soil DNA showed the formation of three bands indicating that [18O] can be used in stable isotope probing. Labeling soil DNA with [18O]water allows identification of newly grown cells or cells that did not divide during the incubation period and avoids artifacts due to spiking the soil with a labeled nutrient. Because actively growing populations are responsible for much of the assimilation of carbon and nitrogen and use of electron donors and acceptors, [18O]water stable isotope probing provides an exciting new approach to link microbial ecology to ecosystem processes such as the carbon cycle.
DE: 0465 Microbiology: ecology, physiology and genomics (4840)
DE: 0470 Nutrients and nutrient cycling (4845, 4850)
DE: 0486 Soils/pedology (1865)
SC: Biogeosciences [B]
MN: Fall Meeting 2005