HR: 0800h
AN: P51D-0956    [Abstracts]
TI: Microbial community composition and respiratory potential of deep, subpermafrost brine in the Canadian Arctic
AU: * Pfiffner, S M
EM: pfiffner@utk.edu
AF: Indiana-Princeton-Tennessee Astrobiology Institute (IPTAI), University of Tennessee 10515 Research Dr., Ste 300, Knoxville, TN 37932 United States
AU: Smithgall, A
EM: asmithga@utk.edu
AF: Indiana-Princeton-Tennessee Astrobiology Institute (IPTAI), University of Tennessee 10515 Research Dr., Ste 300, Knoxville, TN 37932 United States
AU: Parsons, A
EM: aparsons@utk.edu
AF: Indiana-Princeton-Tennessee Astrobiology Institute (IPTAI), University of Tennessee 10515 Research Dr., Ste 300, Knoxville, TN 37932 United States
AU: Gan, M
EM: ygan@utk.edu
AF: Indiana-Princeton-Tennessee Astrobiology Institute (IPTAI), University of Tennessee 10515 Research Dr., Ste 300, Knoxville, TN 37932 United States
AU: White, D C
EM: dwhite1@utk.edu
AF: Indiana-Princeton-Tennessee Astrobiology Institute (IPTAI), University of Tennessee 10515 Research Dr., Ste 300, Knoxville, TN 37932 United States
AU: Onstott, T C
EM: tullis@princeton.edu
AF: IPTAI, Princeton University, Dept. of Geosciences, Princeton, NJ 08544 United States
AU: Pratt, L
EM: prattl@indiana.edu
AF: IPTAI, Indiana University, Dept. of Geological Sciences, Bloomington, IN 47405 United States
AB: Saline fracture waters were collected at depths between 800 and 1200 meters from the Lupin gold mine. Microbial community composition was measured by phospholipid fatty acid analysis. Biomass estimates were from less than 0.1 to 22 pmol/L which converts to 1,700 to 545,000 cells/L. Phospholipid compositions reveal large proportions (24 to 60 mole percent) of monounsatruates, which points to a predominance of Gram negative bacteria. Terminally branched and mid-branched saturates, which indicate Gram positive bacteria, were present at 5-19 mole percent and 0 to 7 mole percent, respectively. The cyclopropyl to monounsaturated fatty acid ratios indicate that the bacterial communities are physiologically stressed. Respiratory potential was estimated by examining the ubiquinone and menaquinone compositions of the water samples. Menaquinone 7 was the most prominent followed by menaquinone 6. Together these two quinones represented 88 to 96 percent of the quinone profile. Ubiquinone 6 represented 0.4 to 11 percent of the quinone profile. The ratio of ubiquinone to menaquinone ranged from 0.003 to 0.066 which indicates that these waters have been exposed to anoxic conditions for a long time.
DE: 0414 Biogeochemical cycles, processes, and modeling (0412, 0793, 1615, 4805, 4912)
DE: 0424 Biosignatures and proxies
DE: 0439 Ecosystems, structure and dynamics (4815)
DE: 0448 Geomicrobiology
DE: 0465 Microbiology: ecology, physiology and genomics (4840)
SC: Planetary Sciences [P]
MN: Fall Meeting 2005