HR: 0800h
AN: V51C-1506    [Abstracts]
TI: Initial Investigations into the Microbial Diversity at Brothers Seamount, Kermadec-Tonga Arc, New Zealand
AU: * Stott, M B
EM: m.stott@gns.cri.nz
AF: Institute of Geological and Nuclear Sciences, 114 Karetoto Rd, Wairakei, 2730 New Zealand
AU: Nakasone, E
EM: enakason@usc.edu
AF: Department of Microbiology, University of Hawaii, 2538 The Mall, Snyder Hall #111, Honolulu, HI 96822 United States
AU: Saito, J
EM: saitoj@hawaii.edu
AF: Department of Microbiology, University of Hawaii, 2538 The Mall, Snyder Hall #111, Honolulu, HI 96822 United States
AU: Dunfield, P
EM: p.dunfield@gns.cri.nz
AF: Institute of Geological and Nuclear Sciences, 114 Karetoto Rd, Wairakei, 2730 New Zealand
AU: Malahoff, A
EM: a.malahoff@gns.cri.nz
AF: Institute of Geological and Nuclear Sciences, 114 Karetoto Rd, Wairakei, 2730 New Zealand
AU: Malahoff, A
EM: a.malahoff@gns.cri.nz
AF: Department of Oceanography, University of Hawaii, 1000 Pope Road, Marine Sciences Building #319, Honolulu, HI 96822 United States
AU: Alam, M
EM: M.Alam@gns.cri.nz
AF: Department of Microbiology, University of Hawaii, 2538 The Mall, Snyder Hall #111, Honolulu, HI 96822 United States
AU: Alam, M
EM: M.Alam@gns.cri.nz
AF: Maui High Performance Computing Center, 550 Lipoa Parkway, Kihei, HI 96753 United States
AB: Brothers Seamount, a submarine volcano located along the Kermadec-Tonga Arc in New Zealand, results from intraoceanic plate subduction and as such, exhibits substantially different chemistries to those found on mid-ocean spreading ridges. Furthermore, Brothers Seamount has two major venting sites that exhibit disparate geochemistries. Combined, these factors suggest that microbial diversity studies of Brothers Volcano have potential to identify an array of novel microbial species. Two manned submarine expeditions, the first in November 2004 with the JAMSTEC R/V Yokosuka and Shinkai 6500 and the second in April/May 2005 with the HURL R/V Kiamikai-o-Kanaloa and Picses V dove on the Brothers Seamount a total of 9 times collecting vent, sediment and fluid samples used, in part, for microbial enrichments and molecular diversity studies. Clone libraries of the 16S rRNA gene were generated using DNA extracts from multiple samples at the two venting sites on Brothers Volcano. So far, more than 60 unique bacterial sequence types have been found out of an overall clone count of greater than 200 including  , ƒ, a, „ and †-Proteobacteria, and several Planctomycetes. The †-Proteobacteria account for most of the diversity. Archaeal 16S rRNA clone banks, including amplifications using primers specific for the phylum Nanoarchaeota, are currently being constructed, with preliminary results showing a diverse range of archaeal species.
DE: 0465 Microbiology: ecology, physiology and genomics (4840)
DE: 8424 Hydrothermal systems (0450, 1034, 3017, 3616, 4832, 8135)
SC: Volcanology, Geochemistry, Petrology [V]
MN: Fall Meeting 2005