HR: 1340h
AN: B33A-0250    [Abstracts]
TI: Atmospheric microbiology in coastal northern California during Asian dust events
AU: * Warren-Rhodes, K A
EM: kwarren-rhodes@mail.arc.nasa.gov
AF: The University of California at Berkeley, Ecosystem Sciences Division, Department of Environmental Science, Policy & Management, 151 Hilgard Hall #3110, Berkeley, CA 94720 United States
AU: * Warren-Rhodes, K A
EM: kwarren-rhodes@mail.arc.nasa.gov
AF: NASA-Ames Research Center, Mail Stop 245-3, Moffett Field, CA 94035 United States
AU: Griffin, D W
EM: dgriffin@usgs.gov
AF: United States Geological Survey, Center for Coastal Geology and Regional Marine Studies, 600 4th St. South, St. Petersburg, FL 33701 United States
AB: Each year, billions of tons of dust are swept from deserts in China and Africa across the globe to the US and Caribbean. Microorganisms are likely hitchhikers aboard this aerosolized dust, with potential human health and ecological impacts. In order to investigate the presence of bacteria and fungi in dust storms from Asia, atmospheric samples for cultivatable microbiological analysis were collected during the NASA Extended- Modis Validation Experiment (EVE), occurring April 21-30, 2004 and coinciding with seasonal Asian dust storm activity. Samples were taken by Twin Otter aircraft along the coast of northern California ($\sim$100 km offshore of Monterey to San Francisco). An $\sim$100 km horizontal leg was flown at $\sim$100 km altitude, typically in the marine boundary layer, followed by a vertical spiral to the dust layer (as indicated by aerosol extinction monitoring) and a second horizontal leg in the dust layer at higher altitudes (2,100-4,200 m). Air samples were taken via Venturi tube inlets with sterile Millipore filter holders outfitted with 47 mm diameter test filters connected to a vacuum pump system. Total sample time varied and was based on flight conditions and EVE objectives. Typical flow rates were 40 lpm and average sample times were $\sim$1hr in the marine layer and $\sim$30 minutes in the dust layer. Control samples for handling and contamination were also obtained. Microbial culture of the filters was conducted using sterile techniques and R2A agar, with filters incubated in the dark at room temperature and monitored for growth over a 2-week period. Fungi and bacterial colonies were further isolated on fresh plates of R2A and Tryptic Soy Broth for the purpose of cataloging/storage. No isolates were obtained from samples of dust layers at altitude. This result may be explained by: i) inadequate sample volumes to detect extremely low bacterial numbers, though sample volumes ranged from 750-2100 liters, ii) light dust layer concentrations during the sampling period and/or iii) problems with the sampling protocol. The latter can be ruled out since isolates were obtained for marine boundary layer samples ($<$100 m). All controls were negative for growth. Bacterial and fungal isolates from the marine layer were identified via 16S and 18S rDNA sequencing and included fungi such as {\it Cladosporium cladosporioides}, a microbe routinely found in atmospheric samples, and bacteria such as {\it Bacillus mycoides}, a nitrogen fixer and common soil isolate.
DE: 0305 Aerosols and particles (0345, 4801)
SC: Biogeosciences [B]
MN: 2004 AGU Fall Meeting