HR: 09:15h
AN: B51D-06    [Abstracts]
TI: Simultaneous Fe, S and P Speciation Using HPLC=HR-ICPMS
AU: Hamester, M
EM: Meike.Hamester@thermo.com
AF: Thermo Electron, Barkhausenstrasse 2, Bremen, 28197 Germany
AU: Lindemann, T
EM: Torsten.Lindemann@thermo.com
AF: Thermo Electron, Barkhausenstrasse 2, Bremen, 28197 Germany
AU: * Rottmann, L
EM: Lothar.Rottmann@thermo.com
AF: Thermo Electron, Barkhausenstrasse 2, Bremen, 28197 Germany
AU: Douthitt, C B
EM: thermochuck@starband.net
AF: Thermo Electron, Barkhausenstrasse 2, Bremen, 28197 Germany
AB: Iron, sulphur and phosphorus-containing biomolecules play important roles in biochemistry and proteomics and are part of the metallome of an organism. Analysis of HPLC peaks by ICPMS for these metals is a difficult analytical challenge because of polyatomic interferences: carbon, oxygen and nitrogen based interferences (NO+, NOH+, COH+, H3CO+, O2+) are the principal limitation in P and S detection and ArO+ is the principal interference on Fe. Polyatomic interferences formed by the elements in the different mobile phases can be separated from P and S in the molecules of interest, allowing the use of gradient elution. With high resolution ICPMS (HR-ICPMS), P and S can be completely resolved from these interferences even in 100 % acetonitrile. Protein phosphorylation can be determined accurately by HR-ICPMS, either on HPLC peaks or by laser ablation of gel electrophoresis spots. The high power magnet field regulator of the Finnigan ELEMENT2 enables rapid mass scanning, e.g. a duty cycle of 99% is obtained for the simultaneous detection of P and S. In order to show the potential of HR-ICPMS for metallomics, mixtures of deoxyribonucleotides (dAMP, dTMP, dGMP, dCMP) and peptides have been separated by HPLC with simultaneous on-line quantitation of P and S, and hemoglobin and myoglobin were analyzed for Fe/S ratios and quantitative determination of Fe.
DE: 8494 Instruments and techniques
SC: Biogeosciences [B]
MN: 2004 AGU Fall Meeting