HR: 1340h
AN: B53C-1004 [Abstracts]
TI: Microbial Sulfur Cycling in an Acid Mine Lake
AU: Bernier, L
EM: berniejm@univmail.cis.mcmaster.ca
AF: School of Geography and Earth Sciences, McMaster University, 1280 Main St. West, Hamilton, ON L8S4K1
Canada
AU: * Warren, L A
EM: warrenl@mcmaster.ca
AF: School of Geography and Earth Sciences, McMaster University, 1280 Main St. West, Hamilton, ON L8S4K1
Canada
AB:
Geochemical dynamics of a tailings impacted lake in Northern Ontario were investigated over a three-year period, in which
active pyrrhotite slurry disposal was initiated in year two. A strong seasonal trend of decreasing epilimnetic pH with
significant diurnal acid production, pre-, during and post slurry deposition was observed with high rates observed compared
to pre-slurry. Slurry deposition occurred at the surface of the lake and acted as a reaction stimulant for acid generation.
Over the diurnal timescale investigated, the highest rates of acid production occurred not at the lake surface but within
the metaliminetic region of the lake. This region was exemplified by strong decreasing oxygen gradients, and thus observed
high rates of acid generation are more consistent with microbial pathways of sulfur oxidation than with abiotic, oxygen
catalyzed pathways. Consistent with microbial catalysis, metalimnetic rates of acid generation were highest during June and
July when microbial populations and metabolic rates were maximal. These results indicate that microbial oxidation of sulfur
species play a major role in acid generation in this system. Further, observed rates of acid generation exceed those
predicted by published abiotic rates of pyrrhotite oxidation, but are consistent with literature estimates of acid generation
catalyzed by microbial activity. Acidithiobacilli accounted for up to 50% of the microbial community pre slurry, but were
absent post slurry deposition. These results are the first to demonstrate quantitatively that microbial sulfur oxidation can
play a predominant role in acid generation within mine tailings impacted systems. They further highlight the need to evaluate
the more complex pathways by which microorganisms process sulfur as the conditions, controls and process rates differ from
those observed for abiotic reactions.
DE: 0330 Geochemical cycles
DE: 0400 Biogeosciences
SC: Biogeosciences [B]
MN: 2004 AGU Fall Meeting