HR: 0800h
AN: V41B-1379    [Abstracts]
TI: Molecular ecological analysis of the distribution and diversity of sulfate-reducing prokaryotes and microbes in deep-sea hydrothermal sites of the Suiyo Seamount, Izu-Bonin Arc, and the Mariana Arc-Backarc, Western Pacific
AU: maruyama, a
EM: maruyama-aki@aist.go.jp
AF: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566 Japan
AU: Nakagawa, T
EM: n-takko@ganko.tohoku.ac.jp
AF: Department of Mineralogy, Petrology and Economic Geology, Graduate School of Science, Tohoku University, Aoba, Aramaki-Aza, Aoba-ku, Sendai, Miyagi, 980-857 Japan
AU: * Hase, Y
EM: hase-yumiko@c.metro-u.ac.jp
AF: Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University, Minami-ohsawa 1-1, Hachioji, Tokyo, 192-0397 Japan
AU: Ishibashi, J
EM: ishi@geo.kyushu-u.ac.jp
AF: Department of Earth and Planetary Science, Faculty of Science, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8581 Japan
AU: Yamanaka, T
EM: tyama@rc.kyushu-u.ac.jp
AF: Graduate School of Social and Cultural Studies, Kyushu University, Ropponmatsu 4-2-1, Chuo-ku, Fukuoka-shi, Fukuoka, 810-8560 Japan
AU: Morimoto, Y
EM: ishi@geo.kyushu-u.ac.jp
AF: Department of Earth and Planetary Science, Faculty of Science, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka-shi, Fukuoka, 812-8581 Japan
AU: Kimura, H
EM: shkimur@ipc.shizuoka.ac.jp
AF: Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Oya 836, Shizuoka-shi, Shizuoka, 422-8529 Japan
AU: Urabe, T
EM: urabe@eps.s.u-tokyo.ac.jp
AF: Department of Earth and Planetary Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
AU: Fukui, M
EM: my-fukui@pop.lowtem.hokudai.ac.jp
AF: The Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido, 060-0819 Japan
AB: The present study describes the distribution and diversity of sulfate-reducing prokaryotes from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, and the Mariana Arc-Backarc Western Pacific. We used a PCR-based metabolic molecular ecology approach that targets a conserved region of subunit A and B of the dissimilatory sulfite reductase (DSR) gene and subunit A of the adenosine-5'-phosphosulfate (APS) reductase gene. The DSR genes were obtained from microbes that grew in catheter-type in situ growth chamber deployed for three days on a vent, and from the effluent water of drilled holes at 5 degree C and natural vent fluids at 7 degree C in the Suiyo. The DSR clones were not closely related to cultivated species or environmental clones. Similarly, novel APS clones were obtained from the mat developed at hydrothermal sites in the Mariana. Moreover, samples of microbial communities from the Suiyo were examined using PCR-denaturing gradient gel electrophoresis (DGGE) analysis based on the 16S rRNA gene. The sequence analysis of 16S rRNA gene fragments obtained from the vent-catheter after a three-day incubation revealed the occurrence of bacterial DGGE bands affiliated with the Aquificae, gamma-, and epsilon-Proteobacteria as well as the occurrence of archaeal phylotypes affiliated with the Thermococcales and of a unique Archaeon sequence clustered with Nanoarchaeota. The DGGE bands obtained from drilled holes and natural vent fluids from 7 to 300 degree C were affiliated with the delta-Proteobacteria, genus Thiomicrospira and Pelodictyon. The dominant DGGE bands retrieved from the effluent water of casing pipes at 3 and 4 degree C were closely related to phylotypes obtained from the Arctic Ocean. Our results suggest the presence of microorganisms corresponding to a unique DSR and APS lineage not detected previously from other geothermal environments.
DE: 8135 Hydrothermal systems (8424)
DE: 4830 Higher marine organisms
DE: 4840 Microbiology
DE: 5455 Origin and evolution
DE: 0400 Biogeosciences
SC: Volcanology, Geochemistry, Petrology [V]
MN: 2004 AGU Fall Meeting