HR: 0800h
AN: V41B-1380    [Abstracts]
TI: Microbial Community in the Hydrothermal System at Suiyo Seamount on the Izu-Bonin Arc
AU: * Yamagishi, A
EM: yamagish@ls.toyaku.ac.jp
AF: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Kasai, H
AF: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Hara, K
AF: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Kakegawa, T
AF: Tohoku University, Graduated School of Science, 6-3 Aoba, Aramaki Aza, Sendai, 980-8578 Japan
AU: Maruyama, A
AF: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8566 Japan
AU: Ishibashi, J
AF: Department of Earth Planetary Sciences, Faculty of Science, Kyushu University, 6-101 Hakozaki, Higashi, 812-8581 Japan
AU: Marumo, K
AF: National Institute of Advanced Industrial Science and Technology (AIST), 1-1-3 No. 7, Tsukuba, 305-8566 Japan
AU: Yamashiro, K
AF: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Itahashi, S
AF: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Urabe, T
AF: Earth and Planetary Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, 113-0033 Japan
AB: Microbial community in hydrothermal area at seafloor has been analyzed by culture-dependent and culture-independent methods. Hydrothermal vent water and vent chimneys have been analyzed by PCR. Hyperthermophilic microbes have been isolated from these environments. Though the analysis of these samples can provide the window to penetrate the microbial community under the seafloor, more direct analysis is desired for better understanding of the sub-seafloor microbial community In the "Archaean Park Project" supported by Special Coordination Fund, several holes were bored and cased in the crater of the Suiyo Seamount on the Izu-Bonin Arc, Japan (about 1,400 m depth) in 2001 and 2002. Hydrothermal fluids were sampled at various sites of cased holea at the Suiyo Seamount. Bacterial clone analysis of water samples collected from some drilled holes was reported by Nakagawa et al. recently. The fluids were filtered to collect the microbial cells. The DNA was extracted and used to amplify archaeal 16S rDNA fragments by PCR using bacterial specific primers. The PCR fragments were cloned and sequenced. Clone-analysis showed significant variation in bacterial sequences found in these samples. The species-patterns suggested that the contamination of ambient seawater to hydrothermal fluid is low. Difference in the dominant species depending on the location was found, suggesting that the bacterial community at sub-sea floor is not monotonous but has gradual shift from the hydrothermal center to peripheral area. Higher number of chemolithoautotrophe-related clones were recovered from the samples collected at central hydrothermal area. Higher number of heterotrophe-related clones were recovered from the samples collected at peripheral area. Results obtained from South Mariana area will be compared. References Takai et al. Genetics 152: 1285-1297 (1999), Takai et al. Appl. Environ. Microbioi. 67: 3618-3629 (2001), Summit et al. Proc. Natl. Acad. Sci. 98: 2158-2163 (2001), Wery et al. FEMS Microbiology Ecology 41: 105-114 (2002) Nakagawa et al. Appl. Environ. Microbiol. 70: 393-403 (2004)
DE: 4815 Ecosystems, structure and dynamics
DE: 4832 Hydrothermal systems
DE: 4840 Microbiology
DE: 4803 Bacteria
DE: 0400 Biogeosciences
SC: Volcanology, Geochemistry, Petrology [V]
MN: 2004 AGU Fall Meeting