HR: 0800h
AN: V41B-1394    [Abstracts]
TI: Microbial Community in the Hydrothermal System at Southern Mariana Trough
AU: * Kato, S
EM: s007029@educ.ls.toyaku.ac.jp
AF: Department of Molecular Biology, Tokyo Univerisity of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Itahashi, S
AF: Department of Molecular Biology, Tokyo Univerisity of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AU: Kakegawa, T
AF: Department of Earth Science, Tohoku University, 6-3 Aoba, Aramaki Aza, Sendai, 980-8578 Japan
AU: Utsumi, M
AF: Institute of Agriculturural and Forest Engineering, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8572 Japan
AU: Maruyama, A
AF: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8566 Japan
AU: Ishibashi, J
AF: Department of Earth Planetary Sciences, Faculty of Science, Kyushu University, 6-101 Hakozaki, Higashi, 812-8581 Japan
AU: Marumo, K
AF: National Institute of Advanced Industrial Science and Technology (AIST), 1-1-3 No. 7, Tsukuba, 305-8566 Japan
AU: Urabe, T
AF: Earth and Planetary Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, 113-0033 Japan
AU: Yamagishi, A
EM: yamagish@ls.toyaku.ac.jp
AF: Department of Molecular Biology, Tokyo Univerisity of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392 Japan
AB: There is unique ecosystem around deep-sea hydrothermal area. Living organisms are supported by chemical free energy provided by the hydrothermal water. The ecosystem is expected to be similar to those in early stage of life history on the earth, when photosynthetic organisms have not emerged. In this study, we have analyzed the microbial diversity in the hydrothermal area at southern Mariana trough. In the "Archaean Park Project" supported by special Coordination Fund, four holes were bored and cased by titanium pipes near hydrothermal vents in the southern Mariana trough in 2004. Hydrothermal fluids were collected from these cased holes and natural vents in this area. Microbial cells were collected by filtering the hydrothermal fluid in situ or in the mother sip. Filters were stored at -80C and used for DNA extraction. Chimneys at this area was also collected and stored at -80C. The filters and chimney samples were crushed and DNA was extracted. DNA samples were used for amplification of 16S rDNA fragments by PCR using archaea specific primers and universal primers. The PCR fragments were cloned and sequenced. These PCR clones of different samples will be compared. We will extend our knowledge about microbiological diversity at Southern Mariana trough to compare the results obtained at other area.
DE: 8424 Hydrothermal systems (8135)
DE: 4815 Ecosystems, structure and dynamics
DE: 4840 Microbiology
DE: 4803 Bacteria
DE: 0400 Biogeosciences
SC: Volcanology, Geochemistry, Petrology [V]
MN: 2004 AGU Fall Meeting