HR: 16:00h
AN: B14A-01 INVITED    [Abstracts]
TI: New Tools For Understanding Microbial Diversity Using High-throughput Sequence Data
AU: * Knight, R
EM: Rob.Knight@Colorado.EDU
AF: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Boulder, CO 80309, United States
AU: Hamady, M
EM: hamady@Colorado.EDU
AF: Department of Computer Science, University of Colorado, Boulder, Boulder, CO 80309, United States
AU: Liu, Z
EM: zongzhi.liu@colorado.edu
AF: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Boulder, CO 80309, United States
AU: Lozupone, C
EM: Catherine.Lozupone@Colorado.EDU
AF: Department of Chemistry and Biochemistry, University of Colorado, Boulder, Boulder, CO 80309, United States
AB: High-throughput sequencing techniques such as 454 are straining the limits of tools traditionally used to build trees, choose OTUs, and perform other essential sequencing tasks. We have developed a workflow for phylogenetic analysis of large-scale sequence data sets that combines existing tools, such as the Arb phylogeny package and the NAST multiple sequence alignment tool, with new methods for choosing and clustering OTUs and for performing phylogenetic community analysis with UniFrac. This talk discusses the cyberinfrastructure we are developing to support the human microbiome project, and the application of these workflows to analyze very large data sets that contrast the gut microbiota with a range of physical environments. These tools will ultimately help to define core and peripheral microbiomes in a range of environments, and will allow us to understand the physical and biotic factors that contribute most to differences in microbial diversity.
DE: 0410 Biodiversity
DE: 0430 Computational methods and data processing
DE: 0439 Ecosystems, structure and dynamics (4815)
DE: 0448 Geomicrobiology
DE: 0465 Microbiology: ecology, physiology and genomics (4840)
SC: Biogeosciences [B]
MN: 2007 Fall Meeting