HR: 1340h
AN: B23D-1612 [Abstracts]
TI: Changes of Soil Aggregate C Isotopes in No-Till Corn Following Bromegrass.
AU: * Follett, R F
EM: ronald.follett@ars.usda.gov
AF: Ronald F. Follett, USDA/ARS, 2150 Centre Ave, Bldg D, Ste 100., Fort Collins, CO 80526-
8119, United States
AU: Varvel, G
EM: Gary.Varvel@ars.usda.gov
AF: Gary Varvel, USDA/ARS, 123 Keim Hall, East Campus
University of Nebraska, Lincoln, NE 68583, United States
AU: Vogel, K P
EM: Ken.Vogel@ars.usda.gov
AF: Kenneth P. Vogel, USDA/ARS, 314 Biochemistry Hall
University of Nebraska
P.O. Box 830739, Lincoln, NE 68583-0737, United States
AB:
This field study is near Ithaca, Nebraska, USA (lat. 41.151, long. 96.401) on a Filbert silt loam (fine, smectitic,
mesic Vertic Argialboll). The site was in bromegrass since 1986. Corn was no-till seeded into the bromegrass
sod in spring 1999. A randomized complete block design with three replicates was used. No-till corn was the
main treatment with nitrogen (N) as subplots. N was broadcast at the start of each growing season at 60 or 120
kg N/ha as NH4NO3. Total biomass was measured by weighing 4.4 m of row in each plot. Soil samples were
obtained in May 1999 (baseline sampling), Sept 1999, June 2000, Oct 2000, Sept 2001, Nov 2002, Sept 2003,
and Oct 2005 from pre-selected areas by removal of plant material from the soil surface and removing the 0-5, 5-
10, and at 4 of the 8 harvests also sampling the 10-30 cm depths with a flat-bladed shovel. Soil bulk densities
were determined on clods from each layer. The moist soil was passed through an 8 mm sieve before air drying
and storing. Aggregate size fractions were obtained with a Yoder wet-aggregate method. Soil size fractions
obtained were > 2, 1, 0.5, 0.25, 0.125, 0.045 and < 0.045 mm. Detritus was floated to the surface and
skimmed off for transfer to a separate container. Aggregates were dried at 55°C, weighed, ground, and analyzed
for total C and N and 13C:12C isotope ratio. Because soil organic carbon (SOC) was labeled with the
bromegrass (C3 plant) isotope signature, then during the 77 months of this experiment the re-labeling of each
fraction and the total SOC with the corn (C4 plant) isotope signature and the amounts of SOC lost from aggregate
size fractions with conversion of the bromegrass sod to no-till corn was measured. During 6.5 years, total SOC
decreased from 21.1, 17.0, and 55.8 t/ha in the 0-5, 5-10, and 10-30 cm depths to 20.1, 16.7, and 55.5 t/ha,
respectively. However the SOC in the < 2, 0.5–2, and < 0.5 mm fractions of the 0 – 5 cm depth changed from
62, 21, and 16 % of the total SOC at the studies beginning to 31, 40, and 29 %, respectively, by the end of 77
months. Weight of SOC from C4 plants was 34.8, 49.8, and 73.2 % of total SOC in the 0–5, 5–10, and 0–30 cm
depths, respectively at the beginning of the study, but after 77 months of no-till corn was 47.3, 59.0, and 71.8 % of
total SOC for these same depths. In summary, it is important to evaluate losses or gains of SOC under
cultivation. Use of the 13C:12C ratios, as influenced by reversing the growing sequence of C3 vs. C4 plants,
allows losses of older SOC from C3 plants (bromegrass) vs. that added by growing C4 plants (corn) to be
determined over time and allows rates of change of the SOC associated with various soil fractions to be
evaluated.
DE: 0402 Agricultural systems
DE: 0428 Carbon cycling (4806)
DE: 0454 Isotopic composition and chemistry (1041, 4870)
DE: 0486 Soils/pedology (1865)
SC: Biogeosciences [B]
MN: 2007 Fall Meeting