HR: 16:45h
AN: B24D-04    [Abstracts]
TI: The Integrated Field-Scale Subsurface Research Challenge Site (IFC) at Rifle, Colorado: Preliminary Results on Microbiological, Geochemical and Hydrologic Processes Controlling Iron Reduction and Uranium Mobility
AU: * Long, P E
EM: philip.long@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Banfield, J
EM: jill@eps.berkeley.edu
AF: University of California, Berkeley, Environmental Science, Berkeley, CA 94720,
AU: Bush, R
EM: rich.bush@gjo.doe.gov
AF: U.S. Department of Energy, Legacy Management, Grand Junction, CO 81503,
AU: Campbell, K
EM: kcampbell@usgs.gov
AF: U.S. Geological Survey, 345 Middlefield Rd., Menlo Park, CA 94025,
AU: Chandler, D P
EM: dchandler@akonni.com
AF: Akonni Biosystems, 401 Rosemont, Frederick, MD 21701,
AU: Davis, J A
EM: jadavis@usgs.gov
AF: U.S. Geological Survey, 345 Middlefield Rd., Menlo Park, CA 94025,
AU: Dayvault, R
EM: rdayvault@gjo.doe.gov
AF: SM Stoller, Inc., 2597 B 3/4 Rd., Grand Junction, CO 81503,
AU: Druhan, J
EM: jennydruhan@berkeley.edu
AF: University of California, Berkeley, Environmental Science, Berkeley, CA 94720,
AU: Elifantz, H
EM: helifant@microbio.umass.edu
AF: University of Massachusetts, Department of Microbiology, Amherst, MA 01003,
AU: Englert, A
EM: alenglert@lbl.gov
AF: Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, CA 94720,
AU: Hettich, R L
EM: hettichrl@ornl.gov
AF: Oak Ridge National Laboratory, 1 Bethel Valley Rd., Oak Ridge, TN 37830,
AU: Holmes, D
EM: dholmes@microbio.umass.edu
AF: University of Massachusetts, Department of Microbiology, Amherst, MA 01003,
AU: Hubbard, S
EM: sshubbard@lbl.gov
AF: Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, CA 94720,
AU: Icenhower, J
EM: jonathan.icenhower@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Jaffe, P R
EM: jaffe@princeton.edu
AF: Princeton University, Dept. of Civil and Environmental Engineering, Princeton, NJ 08544,
AU: Kerkhof, L J
EM: kerkhof@marine.rutgers.edu
AF: Rutgers University, Inst. of Marine and Coastal Sciences, Brunswick, NJ 08901,
AU: Kukkadapu, R K
EM: ravi.kukkadapu@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Lesher, E
EM: elesher@mines.edu
AF: Colorado School of Mines, Div. of Environmental Science and Engineering, Golden, CO 80401,
AU: Lipton, M
EM: mary.lipton@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Lovley, D
EM: dlovley@microbio.umass.edu
AF: University of Massachusetts, Department of Microbiology, Amherst, MA 01003,
AU: Morris, S
EM: sarah.morris@gjo.doe.gov
AF: SM Stoller, Inc., 2597 B 3/4 Rd., Grand Junction, CO 81503,
AU: Morrison, S
EM: stan.morrison@gjo.doe.gov
AF: SM Stoller, Inc., 2597 B 3/4 Rd., Grand Junction, CO 81503,
AU: Mouser, P
EM: pmouser@microbio.umass.edu
AF: University of Massachusetts, Department of Microbiology, Amherst, MA 01003,
AU: Newcomer, D
EM: darrell.newcomer@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: N'Guessan, L
EM: nguesa@microbio.umass.edu
AF: University of Massachusetts, Department of Microbiology, Amherst, MA 01003,
AU: Peacock, A
EM: apeacock@haleyaldrich.com
AF: Microbial Insights, 2340 Stock Creek, Rockford, TN 37853,
AU: Qafoku, N
EM: nik.qafoku@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Qafoku, N
EM: nik.qafoku@pnl.gov
AF: Microbial Insights, 2340 Stock Creek, Rockford, TN 37853,
AU: Resch, C T
EM: tom.resch@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Spane, F
EM: frank.spane@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AU: Spaulding, B
EM: spaldingpb@ornl.gov
AF: Oak Ridge National Laboratory, 1 Bethel Valley Rd., Oak Ridge, TN 37830,
AU: Steefel, C
EM: cisteefel@lbl.gov
AF: Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, CA 94720,
AU: Verberkmoes, N
EM: nve@ornl.gov
AF: Oak Ridge National Laboratory, 1 Bethel Valley Rd., Oak Ridge, TN 37830,
AU: Wilkins, M
EM: mjwilkins@gmail.com
AF: University of California, Berkeley, Environmental Science, Berkeley, CA 94720,
AU: Williams, K H
EM: khwilliams@lbl.gov
AF: Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, CA 94720,
AU: Yabusaki, S B
EM: yabusaki@pnl.gov
AF: Pacific Northwest National Laboratory, Box 999, Richland, WA 99354,
AB: The IFC at Rifle, Colorado was recently funded by the U.S. Department of Energy to address knowledge gaps in 1) geochemical and microbial controls on stimulated U(VI) bioreduction by iron-reducers, 2) U(VI) sorption under Fe-reducing conditions, 3) post-biostimulation U(VI) stability and removal, and 4) rates of natural bioreduction of U(VI). The over-arching goal of the project is to develop a mechanistic understanding of bioreductive and abiotic processes that control uranium mobility targeting new knowledge that can be translated into scientifically defensible flow and reactive transport process models. The Rifle IFC will conduct a focused set of field and lab experiments that use recently developed sciences of proteogenomics and stable isotope probing to track microbial metabolic status during acetate amendment. This information will be linked to changes in Fe redox status and sulfide minerals, with field-scale changes detected by non-invasive hydrogeophysics, including 3-D resistivity tomography. A key goal of the project is to combine abiotic sorption processes under reducing conditions with biotic processes controlling U(VI) reduction. The initial field-scale experiment for the Rifle IFC was conducted during the summer of 2007 with the objectives of collecting simultaneous metagenomic and proteomic samples during acetate amendment and to assess the impact of intentionally decreasing electron donor concentration on the metabolic processes of iron reducers. The 2007 experiment replicated previous field experiments, producing dominance of Geobacter sp. in groundwater within 10 days after the start of acetate amendment. The experiment also confirmed the importance of heterogeneities in controlling the flux of electron donor and the impact of naturally reduced zones on the duration of Fe reduction.
DE: 0412 Biogeochemical kinetics and reaction modeling (0414, 0793, 1615, 4805, 4912)
DE: 0416 Biogeophysics
DE: 0418 Bioremediation
DE: 0448 Geomicrobiology
DE: 1835 Hydrogeophysics
SC: Biogeosciences [B]
MN: 2007 Fall Meeting