HR: 1340h
AN: B33A-0860    [Abstracts]
TI: Characterization of Microbial Communities in Coal Mine Drainage Treatment Systems With Elevated Manganese
AU: * Tan, H
EM: hxt155@psu.edu
AF: Department of Civil and Environmental Engineering The Pennsylvania State University, 212 Sackett Bldg, University Park, PA 16801, United States
AU: Zhang, G
EM: guz2@psu.edu
AF: Department of Civil and Environmental Engineering The Pennsylvania State University, 212 Sackett Bldg, University Park, PA 16801, United States
AU: Burgos, W
EM: wdb3@psu.edu
AF: Department of Civil and Environmental Engineering The Pennsylvania State University, 212 Sackett Bldg, University Park, PA 16801, United States
AB: Sediment samples were collected from two coal mine drainage treatment sites in western Pennsylvania. Both of the sites use constructed limestone beds to passively treat acidic coal mine drainage containing elevated manganese (Mn). Site #1 has influent manganese of 150 mg/L and effluent manganese between 40-100 mg/L. Site #2 has influent manganese of 20 mg/L and effluent manganese of less than 0.5 mg/L. Large quantities of black crusts were deposited throughout the beds at both sites. X-ray diffraction showed these crusts constituted of buserite, which is a layered structure manganese oxide mineral. Both culture-dependent and nucleic acid- based techniques were used to characterize the bacterial and fungal communities in these beds. 16S rRNA gene analysis showed that bacterial communities were very diverse and included Cyanobacter, Proteobacteria, Bacteroidete, Planctomyceta, Acidobacter, Actinobacter and Gemmatimonade taxa. The archaeal diversity was lower and most sequences were related to uncultivated species. Two Mn-oxidizing fungi strains were isolated from one of the sites. One of the fungi is capable of oxidizing Mn(II) at both low and netural pH (3-7) while the other fungi can only oxidze Mn(II) at circumneutral pH. 18S rRNA gene analysis showed the low pH Mn-oxidizing fungus was closely related to Menispora tortuosa, Chaetosphaeria curvispora and Kionochaeta spissa, and the circumneutral Mn-oxidizing fungus was closely related to Myrothecium verrucaria, Didymostilbe echinofibrosa and Myrothecium roridum.
DE: 0419 Biomineralization
DE: 0448 Geomicrobiology
DE: 0463 Microbe/mineral interactions
DE: 0471 Oxidation/reduction reactions (4851)
DE: 0496 Water quality
SC: Biogeosciences [B]
MN: 2007 Fall Meeting