HR: 1330h
AN: NB33M-04    [Abstracts]
TI: Gene Flow Patterns of the Mayfly Fallceon quilleri in San Diego County, California.
AU: * Zickovich, J
EM: zickovich@hotmail.com
AF: San Diego State University, Department of Biology 5500 Campanile Drive , San Diego, CA 92182-4614 United States
AU: Bohonak, A J
EM: bohonak@sciences.sdsu.edu
AF: San Diego State University, Department of Biology 5500 Campanile Drive , San Diego, CA 92182-4614 United States
AB: Management decisions and conservation strategies for freshwater invertebrates critically depend on an understanding of gene flow and genetic structure. We collected the mayfly Fallceon quilleri (Ephemeroptera: Baetidae) from 15 streams across three geographically distinct watersheds in San Diego County, California (San Dieguito, Santa Margarita, and Tijuana) and one site in Anza-Borrego desert. We sequenced a 667 base pair region of the mitochondrial DNA (COI) to assess genetic structure and gene flow. We found eight haplotypes across all populations. San Dieguito and Santa Margarita each contained six haplotypes. Tijuana and Anza Borrego each contained four haplotypes. The expected heterozygosity for San Dieguito, Santa Margarita, Tijuana, and Anza Borrego was 0.81, 0.83, 0.75, and 1.0, respectively. A hierarchical AMOVA analysis indicated restricted gene flow and a pairwise comparison indicated that Tijuana watershed differs significantly from San Dieguito and Anza Borrego. A haplotype cladogram revealed two internal ancestral haplotypes and six derived tip haplotypes that are unique to particular watersheds. These results suggest that Tijuana (the southernmost and the most impacted watershed) is more genetically distinct and isolated than the other watersheds sampled.
DE: 1800 HYDROLOGY
DE: 1899 General or miscellaneous
SC: North American Benthological Society [NB]
MN: 2005 Joint Assembly