HR: 14:15h
AN: U23B-04 [Abstracts]
TI: Autonomous, Near Real-time Detection of Marine Microorganisms using the Environmental Sample Processor (ESP)
AU: * Preston, C
EM: preston@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Marin, R
EM: maro@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: JONES, J
EM: jones@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Jenson, S
EM: sjenson@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Feldman, J
EM: jfeldman@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Massion, E
EM: magene@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Roman, B
EM: brentr@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Cline, D
EM: dcline@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AU: Scholin, C
EM: scholin@mbari.org
AF: Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 95039 United States
AB:
Marine microbes play an essential role in mediating many oceanic biogeochemical cycles. Identification of those microbes as
well as genes and gene products driving such processes, typically require the return of discrete samples to a laboratory.
However, the development and deployment of new classes of ocean observatories create unique opportunities for fielding novel
instruments that eliminate or greatly reduce the need for returning samples to shore for such analyses. In a step towards
realizing this goal, we are exploring use of the Environmental Sample Processor (ESP), a novel instrument that employs a
molecular based assay to detect water borne microorganisms remotely, subsurface, in near real-time. The current ESP
prototype automates application of custom DNA probe arrays to reveal the presence of ecologically relevant microbes and
transmits results of those assays to shore via radio modem. The ESP also archives discrete samples in support of variety of
laboratory-based analyses. Here we discuss the feasibility of using this device to detect selected microbes and key gene
products playing a role oceanic biogeochemical cycles, and consider the applicability of this instrument to studies of
environments ranging from surface waters to deep-sea seeps and hydrothermal vents.
DE: 4803 Bacteria
DE: 4805 Biogeochemical cycles (1615)
DE: 4840 Microbiology
DE: 4894 Instruments and techniques
SC: Union [U]
MN: 2005 Joint Assembly