901303 Cytometer Technologies for Autonomous Platforms : Visit by Jarred Swalwell, Feb 5 2014 This page last changed on Feb 06, 2014 by oreilly. 02/05/2014 /2013 MBARI Jarred Swalwell. Jarred was MBARI seminar speaker. Had discussions with Jarred at 9:00, 12:00, 16:00 9:00 Klimov, Swalwell, O'Reilly Status: new electronics is done but have a noise on analog board from other (switching) board in a board stack. Due to limited resources, now working on components that can be used for existing systems SeaFlow (synergy). Need to solve: Flowcell Modified concept. Laser (test, select) Pump Mechanics. "Unattended" Seaflow operation of a few days is pretty reliable. Generally shorter deployments (few days, a week) compare to CytoBot Rosette profiling pump may be a bit fast for cytometry. Need at least 3 minutes sampling same volume to get good population statistics May come out again in April Flow cell - hoping to use cheap $10 cell, as opposed to IFCB cell which costs $1000s But have to frequently change the cell. Jarred uses MicroPump devices, pretty reliable. Keep clean: consider hydrodynamics near flow cell walls. Testing new cheap 0.5Watt $500 Chinese-made laser - not too stable but can resolve prochlorococus. (ultrabright.com?) They also make $10k laser which is pretty stable so this is matter of price not technology. Could do embedded system with Arduino Camera - would like to take pictures of larger particles. Round table discussion 16:00-17:00 Klimov, O'Reilly, Maughan, Swalwell, Hamilton, Chavez Some of Francois' algorithms can be run in real-time FC: What about machine-learning approaches? E.g. make inferences from clustering and local environment. JS: Often have to specify centroids to Ribalet's algorithms at the beginning. A lot of the clustering algorithms originated from medical applications (Fred Hutchinson Inst.). "Flowcast". Written in 'R', which is free (unlike MathLab) JS: Has done some acoustic applications. Drug-delivery via bubbles, infer bubble material properties from acoustic scatter JS: Note that size of virtual core can be adjusted. Larger VC results in poorer JS: Have processed 2012 data - will provide to Francisco/Jason Fluorescent beads tend to sink; so delivery rate is not constant, but this is not so important as long as some of them appear on cytogram for gating calibration On "real time": 3 min is sort of enough for cytogram, makes 1 file. Real flow rate is 15 mL/min, virtual core flow rate about 15-30 uL / min Plans: Fransico is asking what is good for Jarred: 1st, or 3-4 weeks of April? Jarred: 1st week looks better.

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