901303 Cytometer Technologies for Autonomous Platforms : Imaging Flow CytoBot This page last changed on Sep 09, 2013 by oreilly. Developed by Heidi Sosik and Robert Olson at Woods Hole. HAB and Imaging Flow Cytobot case studies, Sosik's presentation from the CANON Sampling Workshop (2010) Sold by McLane Reseach Labs (here is the McLane specification sheet) Instrument emphasizes imaging - camera/flash-LED is triggered by CHL or SSC PMT pulse height (configurable threshold). ## Performance notes Core consists of seawater flowing at 15 ml/hr = 4.17 x 10^-3 ml/sec. Following PMT pulse, camera/LED may be triggered based on CHL or SSC pulse height and threshold settings. If camera/LED is triggered, subsequent pulses are ignored for at least 80 msec until camera is done (Olson and Sosik 2007). According to Mike Matthewson (McLane): "At 1000 cell/ml [...] IFCB looks at nearly all the sample (i.e., 15 ml/hour [sheath is not sample]); at 5000 cell/ml it looks at ~half of the water passing through the instrument. Reliable concentrations can be obtained up to at least 15,000 cell/ml. Again, the volume of sample actually examined is kept track of and used to calculate cell concentrations." Compute time 'T' to measure N OPP cells at this flow rate 'f', for given values of number density 'p': T = N / (f * p) We assume that cells are not necessarily all the same size, but they are all detectable, and assume that each cell is individually detected (i.e. we don't see multiple cells counted as one).
| N=1000 (sigma=3.1%) | N=500 (sigma=4.4%) | N=100 (sigma=10%)
--- | --- | --- | ---
p=10^2 cell/ml | 2400 s | 1200 s | 240 s
p=10^3 cell/ml | 240 s | 120 s | 24 s
p=10^4 cell/ml |  |  |
p=10^5 cell/ml |  |  |
Attachments: Sosik_HAB_cases.ppt (application/vnd.ms-powerpoint) McLane-IFCB-Datasheet.pdf (application/x-pdf) FlowCytobot-Olson_et_al_DSR2003_8932.pdf (application/x-pdf) IFCB_33704-1.pdf (application/x-pdf) automatedClassification.pdf (application/pdf)

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