901303 Cytometer Technologies for Autonomous Platforms : Technologies overview - 2 This page last changed on Jul 11, 2013 by klimov. Options, features
Method | Notes | Applicability | Adaptability
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Flow path: - Open space - flow cell (quartz, plastic) - Micro fluidics, microchip. Fishman-R | Most bench top flowcytometers use open space path. Flowcytometers for toxic and hazardous substances, and oceanographic use typically enclosed path. | Design of flow path may affect optical performance, sensitivity | Maintenance: - flow path needs to be easily accessible for cleaning or replacement Catastrophic failure (flooding) - flow path needs to be enclosed
Physical volume vs. detectable volume ("virtual core") - SeaFlow, Sealable | Ability to re-process primary data. |  | Pros: - Allows processing of undiluted sample - no need for sheath fluid Cons: - virtual core is harder to characterize then physical volume
Sheath fluid, vs. undiluted sample |  |  |
fluorescent and calibration bits. | Used for calibration on some (most) benchtop flowcytometers |  | Cons: - hard to implement in "in situ" instrument
Cell sorting: - Mechanical sorting into "catcher tube". Enclosed path, one population. - Electrostatic deflection | actively separate and isolate particles having specified properties. | seem to be complex to implement and currently does not required |
Focusing: - Hydrodynamic focusing: coaxial laminar flow - Acoustic focusing (Ward) | This is physical sample focusing |  |
Real time or post processing, or both |  | Real time processing: required Post processing: beneficial |
Labeling - Fluorescent - Staining (Worden - bacterial communities) - Isotope labeling |  |  |
Processing and software - Gating and analyzing software - Population identification using computational methods (rare and hidden populations) - Population identification (Worden): trigger on scattering signal is used, and then accumulate data with other parameters. This is "see everything" approach |  |  |

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