901303 Cytometer Technologies for Autonomous Platforms : Home This page last changed on Sep 11, 2014 by oreilly. This is the home page for the 901303 Cytometer Technologies for Autonomous Platforms space. ### # Background Scientific motivation for in situ autonomous cytometry was described at the 2010 CANON sampling workshop (web page, final report, engineering report), attended by scientists and engineers from multiple institutions. Heidi Sosik from WHOI presented an overview of the Imaging Cytobot instrument and its application for HAB detection. Several breakout groups worked to identify compelling science problems and potential technical approaches to address them. Autonomous cytometry was identified as a key technology by the following breakout groups: Harmful algal blooms and phytoplankton, Zooplankton, Thin layers, and Mesoscale eddies. As a result of this workshop, Worden, Bellingham, Swalwell (UW) and O'Reilly began discussing potential integration of Swalwell's SeaFlow cytometer with LRAUV. MBARI collaborated with UW's Armbrust, Swalwell and others to submit a NASA ASTEP proposal in 2011. We proposed to miniaturize SeaFlow, integrate it with LRAUV, and deploy the system in a Greenland fjord. NASA rated the proposal highly, but was unable to fund it. In 2012, we submitted an internal MBARI proposal that would miniaturize SeaFlow, integrate it with LRAUV, and test the system in Monterey Bay. We proposed that SeaFlow inventor Swalwell participate in a consulting role, providing guidance on approaches to minimize instrument size and power consumption, improve shock protection, etc. Although rated as "likely to be approved" by MBARI management, we were unable to reach agreement with Swalwell on his technical and scientific role. In response we submitted an internal MBARI feasibility study to look at a broad range of commercial and research cytometers which could potentially be integrated with LRAUV and other MBARI autonomous platforms. # Science applications and requirements Taxa tracked in MBARI BOG database (D. Kolber) Potential AUV applications Potential platforms # Existing instruments SeaFlow, SeaLabel CytoSense, CytoBuoy, CytoSub Imaging Flow Cytobot FlowCam Digital Holographic Microscopes Oceanographic cytometers compared with one another and to other methods Some non-oceanographic instruments # Technologies Coulter Counter Cell phone cytometer Cell-substrate Impedance Sensing, Electroporation Optical Detectors Technologies overview - 1 Technologies overview - 2 Technologies overview - 3 (Specifications and parameters)) # Meeting notes Meeting notes Ken Johnson and Gene Massion - Coulter Counter, 9/12/2013 Visit by Heidi Sosik, 9/11/2013 Telecon with Fluid Imaging, 6/12/2013 Telecon with Fluid Imaging, 8/20/2013 Discussion with Jared Swalwell on 6/6/2013 Visit by Jarred Swalwell, seminar speaker on 2/5/2014 Visit by Peter Lopez, 9/10/2014 # Useful links Practical Flow Cytometry (H. Shapiro - the standard text.) Howard Shapiro's web page Flow Cytometry - A Basic Introduction Flow cytometry: retrospective, fundamentals, and recent instrumentation (Cytotechnology, 2012); includes discussion of emerging cytometry technologies Imaging versus flow cytometers (Webinar) http://plankt.oxfordjournals.org/content/30/3/333.full The emergence of automated high-frequency flow cytometryâ?? (Journal of Plankton Research, 2008) Cellular Astronomy: a Foreseeable Future in Cytometry (H. Shapiro, Cytometry, 2004) A Hardware-accelerated Approach for Imaging Flow Cytometry (Lee, Meng, et al, 2013 International Conference on Field Programmable Logic and Applications) MIT 3D Optical Systems Group # Potentially relevant workshops, conferences, trade shows Cytometry Development Workshop, Asilomar - Next workshop is October 23-27, 2013 This is apparently a technology-oriented workshop for experts in the field - perhaps Alex Worden is the only one who would qualify. American Society for cell Biology , conference and expo, mid December annually. www.ascb.org Attachments: ASTEP_2011_v8.pdf (application/x-octect-stream) PhytoplanktonCountingByFlowCytometry.pdf (application/x-octect-stream) emergence-of-automated-high-frequency-flow-cytometry.pdf (application/x-octect-stream) CANONSamplingWorkshopEngineeringReport.pdf (application/force-download) Sosik_HAB_cases_CANON_2010_Workshop.ppt (application/vnd.ms-powerpoint) CANON Sampling Workshop Report.20Apr10.pdf (application/force-download) Flow cytometry - Wikipedia, the free encyclopedia.pdf (application/pdf) 2013-06-06-MeetingNotes-Jarred Swalwell-by-DK.pdf (application/x-pdf) CelluluarAstronomy-Shapiro.pdf (application/x-pdf) HardwareAcceleratedImagingFlowCytometry.pdf (application/x-pdf)

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