901303 Cytometer Technologies for Autonomous Platforms : Visit by Peter Lopez 9-10-2014 This page last changed on Sep 11, 2014 by oreilly. Lopez, O'Reilly, Klimov Centrifugal elutriation - pre-sort particles Sampling statistics problem Partec - flow microscope Denis - showed SM data PL: Could you acoustically control concentration in SM measurement volume, to prevent excessive particles? Round table: Influx sorting discussion with Camille Scholin: interested in particle-field distribution, to guide adaptive sampling CS: Which is more amenable to real-time sampling: scatter/fluor vs morphological? PL: Need to decide size range. Answer is not always flow cytometry. Look at Shapiro's battery-powered microscope, for remote diagnostics JB: How small can a flow cytometer be? PL: lasers are really small now... a lot of cytometers had to be flexible - now more specialized, smaller versions available. HIV/AIDS applications in developing world are driving size/power downward. CS: What are prospects for in situ cell sorting? PL: Will show some slides... chip-based, not electrostatic, but mechanical. Working with Livermore guys FC: Do you sort live material? PL: Yes - sorting effects on living samples are largely unknown - work to be done . Typically pressurized to 60 psi PL: Centrifuging technique seems pretty gentle. Acoustic tech hasn't made it into sorting applications yet. FC: Anyone thought of breaking up cells and analyzing organelles? PL: Done with e.g. tumor cells. Also lyse cells to extract chromosomes, then run through cytometer PL: Can get fluorescent beads that bind to specific target - then measure beads in cytometer. Can get that fluoresce in different colors SS: Can cells be sorted on basis of morphology? CP: Could we sort environmental DNA? PL: Need some kind of particle basis for cytometry TO: Could this be a mass-spec application? PL: Maybe PL: No reason why a flow cytometer can't be very small - power is more challenging Attachments: PeterLopez_MBARI_09102014.pptx (application/vnd.openxmlformats-officedocument.presentationml.presentation)

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